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Antibodies > COL1A2 / Collagen I Alpha 2 > LS-C804657
 
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Catalog Number Size Price
LS-C804657-100 100 µl (1 mg/ml) $379 
LS-C804657-200 200 µl (1 mg/ml) $421 
COL1A2 / Collagen I Alpha 2 Antibody - 1:100 staining human Melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
COL1A2 / Collagen I Alpha 2 Antibody - Staining 293T cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
COL1A2 / Collagen I Alpha 2 Antibody - Western blot analysis of extracts of HepG2 cells using Collagen I a2 antibody.
COL1A2 / Collagen I Alpha 2 Antibody - Western blot analysis of extracts of 293 cells using Collagen I a2 antibody.
COL1A2 / Collagen I Alpha 2 Antibody - Western blot analysis of extracts of mouse muscle using Collagen I a2 antibody.
COL1A2 / Collagen I Alpha 2 Antibody - 1:100 staining human Melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
COL1A2 / Collagen I Alpha 2 Antibody - Staining 293T cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
COL1A2 / Collagen I Alpha 2 Antibody - Western blot analysis of extracts of HepG2 cells using Collagen I a2 antibody.
COL1A2 / Collagen I Alpha 2 Antibody - Western blot analysis of extracts of 293 cells using Collagen I a2 antibody.
COL1A2 / Collagen I Alpha 2 Antibody - Western blot analysis of extracts of mouse muscle using Collagen I a2 antibody.
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Polyclonal Rabbit anti‑Human COL1A2 / Collagen I Alpha 2 Antibody (IHC, IF, WB) LS‑C804657

Polyclonal Rabbit anti‑Human COL1A2 / Collagen I Alpha 2 Antibody (IHC, IF, WB) LS‑C804657

Submit Review LSBio Guarantee
Antibody:
COL1A2 / Collagen I Alpha 2 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C804657-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
COL1A2 / Collagen I Alpha 2 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Collagen I Alpha 2 antibody LS-C804657 is an unconjugated rabbit polyclonal antibody to Collagen I Alpha 2 (COL1A2) from human. It is reactive with human and mouse. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human COL1A2 / Collagen I Alpha 2
Synonyms
COL1A2 | Alpha 2(I)-collagen | Collagen alpha 2(i) | Collagen, type I, alpha 2 | Alpha-2 type I collagen | Type I procollagen | Collagen alpha-2(I) chain | OI4
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About COL1A2 / Collagen I Alpha 2
P08123 NM_000089 NP_000080.2

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Featured Products

COL1A2 / Collagen I Alpha 2 Antibody - Anti-COL1A2 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.
IHC‑plus™ COL1A2 / Collagen I Alpha 2 Antibody (aa471‑520) LS‑B8536
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
COL1A2 / Collagen I Alpha 2 Antibody - Immunohistochemical analysis of Collagen 1 alpha 2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
COL1A2 / Collagen I Alpha 2 Antibody (N‑Terminus) LS‑C352021
Species: Human, Mouse, Rat, Bovine, Dog, Pig, Sheep
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
COL1A2 / Collagen I Alpha 2 Antibody - Immunohistochemical analysis of Collagen 1 alpha 2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
COL1A2 / Collagen I Alpha 2 Antibody (Internal) LS‑C352030
Species: Human, Dog, Rabbit
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation
COL1A2 / Collagen I Alpha 2 Antibody - Western blot of COL1A2 antibody
COL1A2 / Collagen I Alpha 2 Antibody (1‑80 aa, N‑terminal) LS‑C382805
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, ELISA
COL1A2 / Collagen I Alpha 2 Antibody - Immunohistochemistry of paraffin-embedded Human lung cancer using COL1A2 Polyclonal Antibody at dilution of 1:70.
COL1A2 / Collagen I Alpha 2 Antibody LS‑C400572
Species: Human, Mouse, Rat
Applications: IHC, ELISA
Product
COL1A2 / Collagen I Alpha 2 Antibody (clone 7E11) LS‑C139199
Species: Human
Applications: Western blot, ELISA
See all COL1A2 / Collagen I Alpha 2 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/29/2024
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