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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C354949-10 10 blot $395 
Cofilin Antibody - Immunofluorescence analysis of Phospho-Cofilin pSer3 was done on 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-Cofilin pSer3 Rabbit Polyclonal Antibody at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin. Panel d is a merged image showing cytoplasmic and nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
Cofilin Antibody - WB using Phospho-Cofilin pSer3 Antibody
Cofilin Antibody - Immunofluorescence analysis of Phospho-Cofilin pSer3 was done on 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Phospho-Cofilin pSer3 Rabbit Polyclonal Antibody at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin. Panel d is a merged image showing cytoplasmic and nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
Cofilin Antibody - WB using Phospho-Cofilin pSer3 Antibody
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Cofilin Antibody (phospho‑Ser3) LS‑C354949

Cofilin Antibody (phospho‑Ser3) LS‑C354949

Antibody:
Rabbit Polyclonal to Human Cofilin
Application:
IF, WB
Reactivity:
Human, Dog
Format:
Unconjugated, Unmodified
Price
Catalog Number
$395 
LS-C354949-10
10 blot
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human Cofilin
Application:
IF, WB
Reactivity:
Human, Dog
Format:
Unconjugated, Unmodified

Specifications

Description
Cofilin antibody LS-C354949 is an unconjugated rabbit polyclonal antibody to Cofilin from human and dog. Validated for IF and WB.
Target
Human Cofilin
Host
Rabbit
Reactivity
Human, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Immunofluorescence
  • Western blot
Immunogen
The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human cofilin that contains serine 3. The sequence is conserved in mouse and rat.
Epitope
pSer3
Presentation
PBS, pH 7.3, 1 mg/ml BSA, 0.05% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Publications (0)

Reviews (0)

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Requested From: United States
Date Requested: 10/20/2019