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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CNTF Antibody (aa172‑183) LS‑C139712
CNTF antibody LS-C139712 is an unconjugated goat polyclonal antibody to CNTF from human and mouse. Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C139712-100
100 µg
$405
Description
CNTF antibody LS-C139712 is an unconjugated goat polyclonal antibody to CNTF from human and mouse. Validated for Peptide-ELISA and WB.
Target
Human CNTF
Host
Goat
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Rat, Bat, Hamster (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.5 - 2 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:2000)
Immunogen
CNTF antibody was raised against synthetic peptide C-SIHDLRFISSHQ from an internal region of human CNTF (NP_000605.1). Percent identity with other species by BLAST analysis: Human (100%), Gorilla (92%), Gibbon (92%), Rat (92%), Hamster (92%), Panda (92%), Bat (92%), Mouse (83%), Elephant (83%), Dog (83%), Rabbit (83%), Horse (83%), Pig (83%), Nematode (83%).
Blocking Peptide
LS-E27541 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C139712. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa172-183
Specificity
Human CNTF.
Usage
Peptide ELISA: antibody detection limit dilution 1:2000. Western blot: Approx 17+28kD bands observed in Human, Mouse and Rat Brain lysates (calculated MW of 22.9kD according to Human NP_000605.1 and 22.6kD according to Mouse NP_740756.1). The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-2 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About CNTF
P26441 NM_000614 NP_000605.1

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Images

Western blot

CNTF antibody (0.5 ug/ml) staining of Mouse Brain lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
CNTF antibody (0.5 ug/ml) staining of Mouse Brain lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

CNTF antibody (0.5 ug/ml) staining of Mouse Brain lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
CNTF antibody (0.5 ug/ml) staining of Mouse Brain lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

CNTF antibody (0.5 ug/ml) staining of Mouse Brain lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
CNTF antibody (0.5 ug/ml) staining of Mouse Brain lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

CNTF antibody (0.5 ug/ml) staining of Mouse Brain lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
CNTF antibody (0.5 ug/ml) staining of Mouse Brain lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 12/15/2018
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