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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CNGB1 Antibody LS‑C680296
CNGB1 antibody LS-C680296 is an unconjugated rabbit polyclonal antibody to human CNGB1. Validated for ELISA, IF, IHC and WB.
Catalog
Size
Price
LS-C680296-50
50 µg (4.61 mg/ml)
Unavailable
LS-C680296-100
100 µg
Unavailable

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Product Description

CNGB1 antibody LS-C680296 is an unconjugated rabbit polyclonal antibody to human CNGB1. Validated for ELISA, IF, IHC and WB.
About CNGB1
Subunit of cyclic nucleotide-gated (CNG) channels, nonselective cation channels, which play important roles in both visual and olfactory signal transduction. Q14028 NM_001297 NP_001288.3

CNGB1 Antibody, CNG channel 4 Antibody, CNCG2 Antibody, CNCG4 Antibody, CNG-4 Antibody, CNG4. 1 Antibody, CNG4. 3 Antibody, CNCG3L Antibody, CNG channel beta-1 Antibody, CNG4 Antibody, GARP Antibody, Glutamic acid-rich protein 1 Antibody, Glutamic acid-rich protein Antibody, Glutamic-acid-rich protein Antibody, RCNC2 Antibody, RCNCbeta Antibody, RP45 Antibody, CNGB1B Antibody, RCNCb Antibody


Specifications

Target
Human CNGB1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with HRP, Biotin, FITC.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC (1:200 - 1:500)
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:5000)
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CNGB1 antibody was raised against recombinant Human Cyclic nucleotide-gated cation channel beta-1 protein (131-253AA)
Presentation
0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry - Paraffin

Immunohistochemistry Dilution at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry Dilution at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence

Immunofluorescence staining of A549 cells diluted at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunofluorescence staining of A549 cells diluted at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Western blot

Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, 293T whole cell lysate All Lanes: CNGB1 antibody at 4.6µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 140, 71, 33 KDa Observed band size: 140 KDa
Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, 293T whole cell lysate All Lanes: CNGB1 antibody at 4.6µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 140, 71, 33 KDa Observed band size: 140 KDa

Immunohistochemistry - Paraffin

Immunohistochemistry Dilution at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry Dilution at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence

Immunofluorescence staining of A549 cells diluted at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunofluorescence staining of A549 cells diluted at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Western blot

Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, 293T whole cell lysate All Lanes: CNGB1 antibody at 4.6µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 140, 71, 33 KDa Observed band size: 140 KDa
Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, 293T whole cell lysate All Lanes: CNGB1 antibody at 4.6µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 140, 71, 33 KDa Observed band size: 140 KDa

Immunohistochemistry - Paraffin

Immunohistochemistry Dilution at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry Dilution at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence

Immunofluorescence staining of A549 cells diluted at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunofluorescence staining of A549 cells diluted at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Western blot

Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, 293T whole cell lysate All Lanes: CNGB1 antibody at 4.6µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 140, 71, 33 KDa Observed band size: 140 KDa
Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, 293T whole cell lysate All Lanes: CNGB1 antibody at 4.6µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 140, 71, 33 KDa Observed band size: 140 KDa

Immunohistochemistry - Paraffin

Immunohistochemistry Dilution at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Immunohistochemistry Dilution at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence

Immunofluorescence staining of A549 cells diluted at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Immunofluorescence staining of A549 cells diluted at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The Secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Western blot

Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, 293T whole cell lysate All Lanes: CNGB1 antibody at 4.6µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 140, 71, 33 KDa Observed band size: 140 KDa
Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, 293T whole cell lysate All Lanes: CNGB1 antibody at 4.6µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 140, 71, 33 KDa Observed band size: 140 KDa


Requested From: 
Date Requested: 6/25/2018

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