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Antibodies > CML / BCR > LS-C408184
 
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Catalog Number Size Price
LS-C408184-20 20 µl $275 
LS-C408184-100 100 µl $389 
CML / BCR Antibody - Immunohistochemistry of paraffin-embedded human prostate using BCR antibodyat dilution of 1:100 (40x lens).
CML / BCR Antibody - Immunohistochemistry of paraffin-embedded human stomach using BCR antibodyat dilution of 1:100 (40x lens).
CML / BCR Antibody - Western blot analysis of extracts of various cell lines, using BCR antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
CML / BCR Antibody - Immunohistochemistry of paraffin-embedded human prostate using BCR antibodyat dilution of 1:100 (40x lens).
CML / BCR Antibody - Immunohistochemistry of paraffin-embedded human stomach using BCR antibodyat dilution of 1:100 (40x lens).
CML / BCR Antibody - Western blot analysis of extracts of various cell lines, using BCR antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
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Polyclonal Rabbit anti‑Human CML / BCR Antibody (IHC, WB) LS‑C408184

Polyclonal Rabbit anti‑Human CML / BCR Antibody (IHC, WB) LS‑C408184

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Antibody:
CML / BCR Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C408184-20
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CML / BCR Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
BCR antibody LS-C408184 is an unconjugated rabbit polyclonal antibody to BCR (CML) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.
Target
Human CML / BCR
Synonyms
BCR | Acute lymphocytic leukemia | ALL | BCR1 | BCR/FGFR1 chimera protein | Breakpoint cluster region | Chronic myeloid leukemia | D22S11 | CML | D22S662 | Philadelphia chromosome | PHL | FGFR1/BCR chimera protein
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human BCR (NP_004318.3). MVDPVGFAEAWKAQFPDSEPPRMELRSVGDIEQELERCKASIRRLEQEVNQERFRMIYLQTLLAKEKKSYDRQRWGFRRAAQAPDGASEPRASASRPQPAPADGADPPPAEEPEARPDGEGSPGKARPGTARRPGAAASGERDDRGPPASVAALRSNFERIRKGHGQPGADAEKPFYVNVEFHHERGLVKVNDKEVSDRISSLGSQAMQMERKKSQHGAGSSVGDASRPPYRGRSSESSCGVDGDYEDAELNPRFLKDNLIDANGGSRPPWPPLEYQPYQSIYVGGMMEGEGKGPLLRSQ
Specificity
Human CML / BCR
Applications
  • IHC (1:50 - 1:100)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
The predicted MW is 137kDa/142kDa, while the observed MW by Western blot was 150kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CML / BCR
P11274 NM_004327 NP_004318.3

Publications (0)

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Featured Products

CML / BCR Antibody - Immunohistochemical analysis of BCR (pY360) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
CML / BCR Antibody (phospho‑Tyr360) LS‑C358919
Species: Human, Monkey, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
CML / BCR Antibody - Western blot of Bcr phosphorylation expression in COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
CML / BCR Antibody (phospho‑Tyr360) LS‑C416534
Species: Human, Mouse
Applications: IHC, ICC, Immunofluorescence, Western blot
Product
CML / BCR Antibody (300‑380 aa) LS‑C381491
Species: Human, Monkey, Mouse
Applications: IHC, Immunofluorescence, Western blot, ELISA
CML / BCR Antibody - Immunohistochemistry analysis of paraffin-embedded human brain, using Bcr (Phospho-Tyr360) Antibody. The picture on the right is blocked with the phospho peptide.
CML / BCR Antibody (phospho‑Tyr360) LS‑C198681
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
CML / BCR Antibody - Detection of Human BCR by Immunocytochemistry. Sample: FFPE section of human K562 cells (contains the chromosomal translocation, t(9:22) that creates the BCR/ABL fusion gene). Antibody: Affinity purified rabbit anti-BCR used at a dilution of 1:1000 (1 ug/ml). Detection: Green-fluorescent goat anti-rabbit IgG - Alexa Fluor 488 used at a dilution of 1:1000.
CML / BCR Antibody (aa1221‑1271) LS‑C288817
Species: Human
Applications: ICC, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
CML / BCR ELISA Kit
Mouse/Human Phospho-CML / BCR (Tyr360) (Cell-Based Phosphorylation ELISA) ELISA Kit - LS-F1292
Reactivity: Human, Mouse
Range: Positive/Negative
See all CML / BCR Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/28/2024
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