CLIC4 antibody LS-B2383 is an unconjugated goat polyclonal antibody to CLIC4 from human, mouse, rat and other species. Validated for IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Human, Monkey, Mouse, Rat
(tested or 100% immunogen sequence identity)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- IHC - Paraffin (5 µg/ml)
- Immunofluorescence (5 µg/ml)
- Western blot (0.1 - 0.3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
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CLIC4 antibody was raised against peptide with sequence NGLKEEDKEPLIE-C, from the N Terminus of the protein sequence according to NP_039234.1.
- Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B2383. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Immunohistochemistry: LS-B2383 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2383 was determined to be 5 ug/ml.
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.