Claudin 2 antibody LS-B7756 is an unconjugated mouse monoclonal antibody to Claudin 2 (CLDN2) from human, mouse, rat and other species. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Mouse CLDN2 / Claudin 2
Mouse, Human, Rat, Dog
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- IHC - Paraffin (10 µg/ml)
- Western blot
- (applications tested for the base form of this product only)
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A synthetic peptide corresponding to a 26 amino acid sequence at the C-terminus of the mouse Claudin-2 protein.
Reacts with the ~22-23kD Claudin-2 protein. This antibody is confirmed reactive with human, mouse, rat and canine. Reactivity is confirmed from extracts of liver and kidney tissues in all four species as well as MDCK and Caco-2 cell lysates. Reactivity with other species has not been determined.
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Immunofluorescence (Rat): 1-3 ug/ml. Western Blot (Human, mouse, rat, canine): 1-3 ug/ml. Immunohistochemistry (Formalin-fixed paraffin embedded tissues): 1-2 ug/ml; Confirmed with human normal colon, spleen and thyroid, colon and breast cancer tissues. Heat induced epitope retrieval with EDTA buffer, pH 8, is required prior to staining.
PBS, pH 7.4, 0.09% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.