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Antibodies > CLCN4 / CLC-4 > LS-C100107
 
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Catalog Number Size Price
LS-C100107-400 400 µl $393 
CLCN4 / CLC-4 Antibody - Retinae were collected from adult White Leghorn chicken and fixed in the eyecup for two hours in 4% Paraformaldehyde (in PBS). Retinae were then removed from the eyecups and incubated in 30% sucrose (in PBS) overnight. Retinal tissue was embedded and frozen in OCT compound and cut into ~15um sections on a cryotome. Sections were blocked in 5% normal goat serum (in 1%BSA/.1%saponin PBS) for one hour and then incubated at RT with 1:250 (1%BSA/.1% saponin PBS) ClC4 antibody for 1 hour. Sections were then washed in PBS (3X10minutes) and then treated with secondary antibody (1:500 Cy3) for one hour. After another PBS wash series, sections were coverslipped and antibody labeling was visualized at 20X with a Leica upright microscope using a TRITC filter set and Xenon LAMP illumination. (Crousillac et al, 2003)
CLCN4 / CLC-4 Antibody - Immunofluorescence image of cultured chick retinal amacrine (neuronal) cells labeled with CLC4 Antibody. Data courtesy of Emily McMains, Louisiana State University.
CLCN4 / CLC-4 Antibody - Western blot of chicken brain tissue incubated with CLC4 Antibody. Data courtesy of Emily McMains, Louisiana State University.
CLCN4 / CLC-4 Antibody - Retinae were collected from adult White Leghorn chicken and fixed in the eyecup for two hours in 4% Paraformaldehyde (in PBS). Retinae were then removed from the eyecups and incubated in 30% sucrose (in PBS) overnight. Retinal tissue was embedded and frozen in OCT compound and cut into ~15um sections on a cryotome. Sections were blocked in 5% normal goat serum (in 1%BSA/.1%saponin PBS) for one hour and then incubated at RT with 1:250 (1%BSA/.1% saponin PBS) ClC4 antibody for 1 hour. Sections were then washed in PBS (3X10minutes) and then treated with secondary antibody (1:500 Cy3) for one hour. After another PBS wash series, sections were coverslipped and antibody labeling was visualized at 20X with a Leica upright microscope using a TRITC filter set and Xenon LAMP illumination. (Crousillac et al, 2003)
CLCN4 / CLC-4 Antibody - Immunofluorescence image of cultured chick retinal amacrine (neuronal) cells labeled with CLC4 Antibody. Data courtesy of Emily McMains, Louisiana State University.
CLCN4 / CLC-4 Antibody - Western blot of chicken brain tissue incubated with CLC4 Antibody. Data courtesy of Emily McMains, Louisiana State University.
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Polyclonal Rabbit anti‑Human CLCN4 / CLC‑4 Antibody (aa663‑689, IF, WB) LS‑C100107

Polyclonal Rabbit anti‑Human CLCN4 / CLC‑4 Antibody (aa663‑689, IF, WB) LS‑C100107

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Antibody:
CLCN4 / CLC-4 Rabbit anti-Human Polyclonal (aa663-689) Antibody
Application:
IF, WB
Reactivity:
Human, Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C100107-400
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CLCN4 / CLC-4 Rabbit anti-Human Polyclonal (aa663-689) Antibody
Application:
IF, WB
Reactivity:
Human, Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
CLC-4 antibody LS-C100107 is an unconjugated rabbit polyclonal antibody to CLC-4 (CLCN4) (aa663-689) from human. It is reactive with human and chicken. Validated for IF and WB.
Target
Human CLCN4 / CLC-4
Synonyms
CLCN4 | ClC-4 | Chloride channel 4 | Chloride channel protein 4 | Chloride transporter ClC-4 | ClC-4A | CLC4
Host
Rabbit
Reactivity
Human, Chicken (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Epitope
aa663-689
Specificity
This CLC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 663-689 amino acids from the C-terminal region of human CLC4.
Applications
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CLCN4 / CLC-4
P51793 NM_001830 NP_001821.2

Publications (0)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/29/2024
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