(tested or 100% immunogen sequence identity)
- Western blot (1:1000 - 1:5000)
- ELISA (1:10000 - 1:50000)
ClC-5 / CLCN5 antibody was raised against a 13 AA Peptide sequence near the C-terminus of rat CLC-5 (1). The peptide was coupled to KLH.
The 13 AA CLC51-P control peptide is 100% conserved in rat and human CLC-5. No significant sequence homology is detected with other CLCs or other proteins. Actual cross-reactivity of antibodies in various species has not been studied. There is a very high degree of protein homology among CLC-3, CLC-4 and CLC-5. However, CLC51-P peptide has very little sequence homology with CLC-3 and CLC-4. Anti-rat CLC41 antibodies were checked against the corresponding CLC-3 and CLC-4. These peptides are also available to confirm specificity of antibodies.
Suitable for use in ELISA, Western Blot. Western Blot: 1:1000-1:5000 for neat serum and 1-10 ug/ml for affinity pure antibody using ECL. Antibodies made to various CLC-5 peptides have been shown to detect a protein of approx. 80kD in kidney. ELISA:1:10000-1:50000 for neat serum and 0.5-1 ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1 ug/ml.
PBS, 0.05% sodium azide.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
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