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Catalog Number Size Price
LS-C777110-100 100 µg (1 mg/ml) $479 
Choline Acetyltransferase Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Choline Acetyltransferase Antibody (D) Composite.
Choline Acetyltransferase Antibody - Western blot analysis of Mouse Brain showing detection of ~82.5kDa Choline Acetyltransferase protein using Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Mouse Brain (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~82.5kDa.
Choline Acetyltransferase Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Choline Acetyltransferase Antibody (D) Composite.
Choline Acetyltransferase Antibody - Western blot analysis of Mouse Brain showing detection of ~82.5kDa Choline Acetyltransferase protein using Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Mouse Brain (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~82.5kDa.
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Polyclonal Rabbit anti‑Human Choline Acetyltransferase Antibody (APC, IF, WB) LS‑C777110

Polyclonal Rabbit anti‑Human Choline Acetyltransferase Antibody (APC, IF, WB) LS‑C777110

Antibody:
Choline Acetyltransferase Rabbit anti-Human Polyclonal (APC) Antibody
Application:
IF, WB
Reactivity:
Human, Mouse
Format:
Allophycocyanin, Unmodified
Other formats:
Price
Catalog Number
$479
LS-C777110-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Choline Acetyltransferase Rabbit anti-Human Polyclonal (APC) Antibody
Application:
IF, WB
Reactivity:
Human, Mouse
Format:
Allophycocyanin, Unmodified
Other formats:

Specifications

Description
Choline Acetyltransferase antibody LS-C777110 is an APC-conjugated rabbit polyclonal antibody to Choline Acetyltransferase from human. It is reactive with human and mouse. Validated for IF and WB.
Target
Human Choline Acetyltransferase
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Allophycocyanin. Also available conjugated with AP, Atto 390, Atto 488, Atto 594, Biotin, FITC, PerCP, RPE.
Purification
Peptide Affinity Purified
Modifications
Unmodified
Immunogen
Synthetic peptide from the N-Terminus to the mid-protein of human Choline O-Acetyltrasferase
Specificity
Predicted molecular weight at ~82.5 kDa. Observed molecular weights between 68-70 kDa.
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Applications tested for the base form of this product only
Usage
Applications should be user optimized.
Presentation
PBS, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Choline Acetyltransferase

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 2/24/2024