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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CHAF1A / CAF1 Antibody (aa500‑550) LS‑C288276
CAF1 antibody LS-C288276 is an unconjugated rabbit polyclonal antibody to human CAF1 (CHAF1A). Validated for IF, IHC, IP and WB.
Catalog
Size
Price
LS-C288276-100
100 µl (0.2 mg/ml)
Unavailable

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Detection of Human Caf1p150 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma Peyer's patch. Antibody: Affinity purified rabbit anti-Caf1p150 used at a dilution of 1:200 (1 ug/ml).
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Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, Proximity Ligation Assay
Antibody
Species: Human
Applications: Western blot, ELISA

Product Description

CAF1 antibody LS-C288276 is an unconjugated rabbit polyclonal antibody to human CAF1 (CHAF1A). Validated for IF, IHC, IP and WB.
About CHAF1A / CAF1
Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. Q13111 NM_005483 NP_005474.2

CHAF1A Antibody, CAF-I p150 Antibody, CAF1P150 Antibody, CAF-1 subunit A Antibody, CAF-1 Antibody, Hp150 Antibody, p150 Antibody, CAF Antibody, CAF-I 150 kDa subunit Antibody, CAF1 Antibody, CAF1B Antibody


Specifications

Target
Human CHAF1A / CAF1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:500)
  • Immunofluorescence (1:50 - 1:500)
  • Western blot (1:2000 - 1:10000)
  • Immunoprecipitation (2 - 5 µg/mg lysate)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Epitope
aa500-550
Specificity
Region between residue 500 and 550 of human chromatin assembly factor 1, subunit A (p150) using the numbering given in entry AAA76736.1 (GeneID 10036).
Usage
Immunohistochemistry: Antigen retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Human controls: Colon Carcinoma.
Manufacturer
Bethyl Laboratories, Inc.
Presentation
Tris-buffered saline, 0.1% BSA, 0.09% sodium azide.
Storage
Store at 2-8°C for up to 1 year.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunohistochemistry

Detection of Human Caf1p150 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma Peyer's patch. Antibody: Affinity purified rabbit anti-Caf1p150 used at a dilution of 1:200 (1 ug/ml).
Detection of Human Caf1p150 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma Peyer's patch. Antibody: Affinity purified rabbit anti-Caf1p150 used at a dilution of 1:200 (1 ug/ml).

Immunohistochemistry

Detection of Human Caf1p150 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma Peyer's patch. Antibody: Affinity purified rabbit anti-Caf1p150 used at a dilution of 1:200 (1 ug/ml).
Detection of Human Caf1p150 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma Peyer's patch. Antibody: Affinity purified rabbit anti-Caf1p150 used at a dilution of 1:200 (1 ug/ml).

Immunohistochemistry

Detection of Human Caf1p150 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma Peyer's patch. Antibody: Affinity purified rabbit anti-Caf1p150 used at a dilution of 1:200 (1 ug/ml).
Detection of Human Caf1p150 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma Peyer's patch. Antibody: Affinity purified rabbit anti-Caf1p150 used at a dilution of 1:200 (1 ug/ml).

Immunohistochemistry

Detection of Human Caf1p150 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma Peyer's patch. Antibody: Affinity purified rabbit anti-Caf1p150 used at a dilution of 1:200 (1 ug/ml).
Detection of Human Caf1p150 by Immunohistochemistry. Sample: FFPE section of human colon carcinoma Peyer's patch. Antibody: Affinity purified rabbit anti-Caf1p150 used at a dilution of 1:200 (1 ug/ml).


Requested From: 
Date Requested: 6/22/2018

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