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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C662700-100 100 µg $355 
CFD / Factor D / Adipsin Antibody - IHC analysis of Factor D using anti-Factor D antibody. Factor D was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Factor D Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CFD / Factor D / Adipsin Antibody - Immunohistochemistry - Anti-Factor D Picoband antibody
CFD / Factor D / Adipsin Antibody - Immunohistochemistry - Anti-Factor D Picoband antibody
CFD / Factor D / Adipsin Antibody - Western blot - Anti-Factor D Picoband antibody
CFD / Factor D / Adipsin Antibody - Western blot analysis of Factor D using anti-Factor D antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat RH35 cell lysates, Lane 2: rat PC-12 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Factor D antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Factor D at approximately 27KD. The expected band size for Factor D is at 27KD.
CFD / Factor D / Adipsin Antibody - IHC analysis of Factor D using anti-Factor D antibody. Factor D was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Factor D Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CFD / Factor D / Adipsin Antibody - Immunohistochemistry - Anti-Factor D Picoband antibody
CFD / Factor D / Adipsin Antibody - Immunohistochemistry - Anti-Factor D Picoband antibody
CFD / Factor D / Adipsin Antibody - Western blot - Anti-Factor D Picoband antibody
CFD / Factor D / Adipsin Antibody - Western blot analysis of Factor D using anti-Factor D antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat RH35 cell lysates, Lane 2: rat PC-12 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Factor D antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Factor D at approximately 27KD. The expected band size for Factor D is at 27KD.
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Polyclonal CFD / Factor D / Adipsin Antibody (IHC, WB) LS‑C662700

Polyclonal CFD / Factor D / Adipsin Antibody (IHC, WB) LS‑C662700

Antibody:
Rabbit Polyclonal to Rat CFD / Factor D / Adipsin
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$355 
LS-C662700-100
100 µg
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Rat CFD / Factor D / Adipsin
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Adipsin antibody LS-C662700 is an unconjugated rabbit polyclonal antibody to rat Adipsin (CFD / Factor D ). Validated for ELISA, IHC and WB.
Target
Rat CFD / Factor D / Adipsin
Synonyms
CFD | ADIPSIN | C3 convertase activator | Complement factor D | Complement protein D | Complement factor D (adipsin) | DF | ADN | Factor D | PFD | Properdin factor D
Host
Rabbit
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
E. coli-derived rat Factor D recombinant protein (Position: I26-A263).
Presentation
4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3 per 100ug antibody
Reconstitution
Reconstitute with 0.2ml distilled H2O.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CFD / Factor D / Adipsin
P00746 NM_001928 NP_001919.2

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Requested From: United States
Date Requested: 2/18/2020