Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C662700-10 10 µg $318 
LS-C662700-100 100 µg $470 
CFD / Factor D / Adipsin Antibody - IHC analysis of Factor D using anti-Factor D antibody. Factor D was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Factor D Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CFD / Factor D / Adipsin Antibody - Immunohistochemistry - Anti-Factor D Picoband antibody
CFD / Factor D / Adipsin Antibody - Immunohistochemistry - Anti-Factor D Picoband antibody
CFD / Factor D / Adipsin Antibody - Western blot analysis of Factor D using anti-Factor D antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat RH35 cell lysates, Lane 2: rat PC-12 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Factor D antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Factor D at approximately 27KD. The expected band size for Factor D is at 27KD.
CFD / Factor D / Adipsin Antibody - Western blot - Anti-Factor D Picoband antibody
CFD / Factor D / Adipsin Antibody - IHC analysis of Factor D using anti-Factor D antibody. Factor D was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Factor D Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CFD / Factor D / Adipsin Antibody - Immunohistochemistry - Anti-Factor D Picoband antibody
CFD / Factor D / Adipsin Antibody - Immunohistochemistry - Anti-Factor D Picoband antibody
CFD / Factor D / Adipsin Antibody - Western blot analysis of Factor D using anti-Factor D antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat RH35 cell lysates, Lane 2: rat PC-12 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Factor D antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Factor D at approximately 27KD. The expected band size for Factor D is at 27KD.
CFD / Factor D / Adipsin Antibody - Western blot - Anti-Factor D Picoband antibody
1 of 5
2 of 5
3 of 5
4 of 5
5 of 5

Polyclonal Rabbit anti‑Rat CFD / Factor D / Adipsin Antibody (IHC, WB) LS‑C662700

Polyclonal Rabbit anti‑Rat CFD / Factor D / Adipsin Antibody (IHC, WB) LS‑C662700

Antibody:
CFD / Factor D / Adipsin Rabbit anti-Rat Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662700-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CFD / Factor D / Adipsin Rabbit anti-Rat Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Adipsin antibody LS-C662700 is an unconjugated rabbit polyclonal antibody to rat Adipsin (CFD / Factor D). Validated for ELISA, IHC and WB.
Target
Rat CFD / Factor D / Adipsin
Synonyms
CFD | ADIPSIN | C3 convertase activator | Complement factor D | Complement protein D | Complement factor D (adipsin) | DF | ADN | Factor D | PFD | Properdin factor D
Host
Rabbit
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Immunogen
E. coli-derived rat Factor D recombinant protein (Position: I26-A263).
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CFD / Factor D / Adipsin
P00746 NM_001928 NP_001919.2

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/26/2024