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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C761125-30 30 µl (1 mg/ml) $215 
LS-C761125-100 100 µl (1 mg/ml) $265 
LS-C761125-200 200 µl (1 mg/ml) $325 
CEBPD + CEBPE Antibody - Immunohistochemical analysis of C/EBP epsilon (pT74) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CEBPD + CEBPE Antibody - Immunofluorescent analysis of C/EBP epsilon (pT74) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.
CEBPD + CEBPE Antibody - Western blot analysis of C/EBP epsilon (pT74) expression in Hela (A), mouse liver (B), Raw264.7 (C), rat liver (D) whole cell lysates.
CEBPD + CEBPE Antibody - Immunohistochemical analysis of C/EBP epsilon (pT74) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
CEBPD + CEBPE Antibody - Immunofluorescent analysis of C/EBP epsilon (pT74) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.
CEBPD + CEBPE Antibody - Western blot analysis of C/EBP epsilon (pT74) expression in Hela (A), mouse liver (B), Raw264.7 (C), rat liver (D) whole cell lysates.
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CEBPD + CEBPE Antibody LS‑C761125

CEBPD + CEBPE Antibody LS‑C761125

Antibody:
Rabbit Polyclonal to Human CEBPD + CEBPE
Application:
IHC, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Sheep
Format:
Unconjugated, Unmodified
Price
Catalog Number
$215 
LS-C761125-30
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human CEBPD + CEBPE
Application:
IHC, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Sheep
Format:
Unconjugated, Unmodified

Specifications

Description
CEBPD + CEBPE antibody LS-C761125 is an unconjugated rabbit polyclonal antibody to CEBPD + CEBPE from human, mouse, rat and other species. Validated for ICC, IF, IHC and WB.
Target
Human CEBPD + CEBPE
Host
Rabbit
Reactivity
Human, Mouse, Rat, Sheep (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Applications
  • IHC (1:50 - 1:100)
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human C/EBP epsilon. The exact sequence is proprietary.
Specificity
Recognizes endogenous levels of C/EBP epsilon (pT74) protein.
Usage
Applications should be user optimized.
Presentation
0.42% Potassium Phosphate, pH 7.3, 0.87% NaCl, 0.01% Sodium Azide, 30% Glycerol
Storage
Upon delivery, aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 10/19/2019