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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CDK7 Antibody (C‑Terminus) LS‑C287296
CDK7 antibody LS-C287296 is an unconjugated rabbit polyclonal antibody to CDK7 from human, mouse and rat. Validated for IP, PLA and WB.
Catalog
Size
Price
LS-C287296-100
100 µl (1 mg/ml)
$395

Popular CDK7 Products

Anti-CDK7 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7848 dilution 1:200.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
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Species: Human
Applications: ICC, Western blot, Immunoprecipitation, Flow Cytometry
Immunohistochemistry of paraffin-embedded human breast cancer at dilution of 1:100
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, ELISA
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-CDK7 mAb.
Species: Human
Applications: ICC, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, Dot Blot
Antibody
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, ICC, Immunofluorescence, Western blot

Product Description

CDK7 antibody LS-C287296 is an unconjugated rabbit polyclonal antibody to CDK7 from human, mouse and rat. Validated for IP, PLA and WB.

Specifications

Target
Human CDK7
Synonyms
CDK7, 39 kDa protein kinase, CDK-activating kinase, CDK-activating kinase 1, CDKN7, Cell division protein kinase 7, Cyclin-dependent kinase 7, HCAK, Protein kinase, Mpk-7, Serine/threonine kinase stk1, CAK, CAK1, Crk4, Kinase subunit of CAK, MO15, p39 Mo15, p39MO15, STK1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:1000 - 1:15000)
  • Immunoprecipitation
  • Proximity Ligation Assay (1:1000 - 1:20000)
Epitope
C-Terminus
Specificity
Region between residues 300 and the C-terminus (residue 346) of human Cyclin-Dependent Kinase 7 using the numbering given in Swiss-Prot entry P50613 (GeneID 1022).
Manufacturer
Bethyl Laboratories, Inc.
Presentation
Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
Storage
Store at 2°C to 8°C for up to 1 year.
Restrictions
For research use only.
About CDK7
Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. P50613 NM_001799 NP_001790.1


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Images

Western blot

Detection of Mouse and Rat CDK7 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-CDK7 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of Mouse and Rat CDK7 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-CDK7 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Western blot

Detection of Human CDK7 by Western Blot and Immunoprecipitation. Samples: RIPA lysate (5 and 25 ug/lane in A; 5 mg in B) from HeLa cells. Antibody: Affinity purified rabbit anti-CDK7 antibody used at 0.1 ug/ml for WB and at 4 ug/5 mg lysate for IP. Detection: Chemiluminescence with an exposure time of 5 minutes in A. Coomassie stain in B. The identity of the band that is CDK7 was verified by mass spectrometry.
Detection of Human CDK7 by Western Blot and Immunoprecipitation. Samples: RIPA lysate (5 and 25 ug/lane in A; 5 mg in B) from HeLa cells. Antibody: Affinity purified rabbit anti-CDK7 antibody used at 0.1 ug/ml for WB and at 4 ug/5 mg lysate for IP. Detection: Chemiluminescence with an exposure time of 5 minutes in A. Coomassie stain in B. The identity of the band that is CDK7 was verified by mass spectrometry.

Western blot

Detection of Mouse and Rat CDK7 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-CDK7 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of Mouse and Rat CDK7 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-CDK7 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Western blot

Detection of Human CDK7 by Western Blot and Immunoprecipitation. Samples: RIPA lysate (5 and 25 ug/lane in A; 5 mg in B) from HeLa cells. Antibody: Affinity purified rabbit anti-CDK7 antibody used at 0.1 ug/ml for WB and at 4 ug/5 mg lysate for IP. Detection: Chemiluminescence with an exposure time of 5 minutes in A. Coomassie stain in B. The identity of the band that is CDK7 was verified by mass spectrometry.
Detection of Human CDK7 by Western Blot and Immunoprecipitation. Samples: RIPA lysate (5 and 25 ug/lane in A; 5 mg in B) from HeLa cells. Antibody: Affinity purified rabbit anti-CDK7 antibody used at 0.1 ug/ml for WB and at 4 ug/5 mg lysate for IP. Detection: Chemiluminescence with an exposure time of 5 minutes in A. Coomassie stain in B. The identity of the band that is CDK7 was verified by mass spectrometry.

Western blot

Detection of Mouse and Rat CDK7 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-CDK7 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of Mouse and Rat CDK7 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-CDK7 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Western blot

Detection of Human CDK7 by Western Blot and Immunoprecipitation. Samples: RIPA lysate (5 and 25 ug/lane in A; 5 mg in B) from HeLa cells. Antibody: Affinity purified rabbit anti-CDK7 antibody used at 0.1 ug/ml for WB and at 4 ug/5 mg lysate for IP. Detection: Chemiluminescence with an exposure time of 5 minutes in A. Coomassie stain in B. The identity of the band that is CDK7 was verified by mass spectrometry.
Detection of Human CDK7 by Western Blot and Immunoprecipitation. Samples: RIPA lysate (5 and 25 ug/lane in A; 5 mg in B) from HeLa cells. Antibody: Affinity purified rabbit anti-CDK7 antibody used at 0.1 ug/ml for WB and at 4 ug/5 mg lysate for IP. Detection: Chemiluminescence with an exposure time of 5 minutes in A. Coomassie stain in B. The identity of the band that is CDK7 was verified by mass spectrometry.

Western blot

Detection of Mouse and Rat CDK7 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-CDK7 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of Mouse and Rat CDK7 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-CDK7 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Western blot

Detection of Human CDK7 by Western Blot and Immunoprecipitation. Samples: RIPA lysate (5 and 25 ug/lane in A; 5 mg in B) from HeLa cells. Antibody: Affinity purified rabbit anti-CDK7 antibody used at 0.1 ug/ml for WB and at 4 ug/5 mg lysate for IP. Detection: Chemiluminescence with an exposure time of 5 minutes in A. Coomassie stain in B. The identity of the band that is CDK7 was verified by mass spectrometry.
Detection of Human CDK7 by Western Blot and Immunoprecipitation. Samples: RIPA lysate (5 and 25 ug/lane in A; 5 mg in B) from HeLa cells. Antibody: Affinity purified rabbit anti-CDK7 antibody used at 0.1 ug/ml for WB and at 4 ug/5 mg lysate for IP. Detection: Chemiluminescence with an exposure time of 5 minutes in A. Coomassie stain in B. The identity of the band that is CDK7 was verified by mass spectrometry.

Requested From: United States
Date Requested: 10/16/2018

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