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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CDK7 Antibody (C-Terminus) for WB/Western, IP LS-C287296

CDK7 antibody LS-C287296 is an unconjugated rabbit polyclonal antibody to CDK7 from human, mouse and rat. Validated for IP, PLA and WB.
Catalog
Size
Price
LS-C287296-100
100 µl (1 mg/ml)
$395
Description
CDK7 antibody LS-C287296 is an unconjugated rabbit polyclonal antibody to CDK7 from human, mouse and rat. Validated for IP, PLA and WB.
Target
Human CDK7
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:1000 - 1:15000)
  • Immunoprecipitation
  • Proximity Ligation Assay (1:1000 - 1:20000)
Epitope
C-Terminus
Specificity
Region between residues 300 and the C-terminus (residue 346) of human Cyclin-Dependent Kinase 7 using the numbering given in Swiss-Prot entry P50613 (GeneID 1022).
Manufacturer
Bethyl Laboratories, Inc.
Presentation
Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
Storage
Store at 2°C to 8°C for up to 1 year.
Restrictions
For research use only.
About CDK7
P50613 NM_001799 NP_001790.1

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Images

Western blot

Detection of Mouse and Rat CDK7 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-CDK7 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of Mouse and Rat CDK7 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-CDK7 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Western blot

Detection of Human CDK7 by Western Blot and Immunoprecipitation. Samples: RIPA lysate (5 and 25 ug/lane in A; 5 mg in B) from HeLa cells. Antibody: Affinity purified rabbit anti-CDK7 antibody used at 0.1 ug/ml for WB and at 4 ug/5 mg lysate for IP. Detection: Chemiluminescence with an exposure time of 5 minutes in A. Coomassie stain in B. The identity of the band that is CDK7 was verified by mass spectrometry.
Detection of Human CDK7 by Western Blot and Immunoprecipitation. Samples: RIPA lysate (5 and 25 ug/lane in A; 5 mg in B) from HeLa cells. Antibody: Affinity purified rabbit anti-CDK7 antibody used at 0.1 ug/ml for WB and at 4 ug/5 mg lysate for IP. Detection: Chemiluminescence with an exposure time of 5 minutes in A. Coomassie stain in B. The identity of the band that is CDK7 was verified by mass spectrometry.

Western blot

Detection of Mouse and Rat CDK7 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-CDK7 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of Mouse and Rat CDK7 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-CDK7 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Western blot

Detection of Human CDK7 by Western Blot and Immunoprecipitation. Samples: RIPA lysate (5 and 25 ug/lane in A; 5 mg in B) from HeLa cells. Antibody: Affinity purified rabbit anti-CDK7 antibody used at 0.1 ug/ml for WB and at 4 ug/5 mg lysate for IP. Detection: Chemiluminescence with an exposure time of 5 minutes in A. Coomassie stain in B. The identity of the band that is CDK7 was verified by mass spectrometry.
Detection of Human CDK7 by Western Blot and Immunoprecipitation. Samples: RIPA lysate (5 and 25 ug/lane in A; 5 mg in B) from HeLa cells. Antibody: Affinity purified rabbit anti-CDK7 antibody used at 0.1 ug/ml for WB and at 4 ug/5 mg lysate for IP. Detection: Chemiluminescence with an exposure time of 5 minutes in A. Coomassie stain in B. The identity of the band that is CDK7 was verified by mass spectrometry.

Western blot

Detection of Mouse and Rat CDK7 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-CDK7 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of Mouse and Rat CDK7 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-CDK7 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Western blot

Detection of Human CDK7 by Western Blot and Immunoprecipitation. Samples: RIPA lysate (5 and 25 ug/lane in A; 5 mg in B) from HeLa cells. Antibody: Affinity purified rabbit anti-CDK7 antibody used at 0.1 ug/ml for WB and at 4 ug/5 mg lysate for IP. Detection: Chemiluminescence with an exposure time of 5 minutes in A. Coomassie stain in B. The identity of the band that is CDK7 was verified by mass spectrometry.
Detection of Human CDK7 by Western Blot and Immunoprecipitation. Samples: RIPA lysate (5 and 25 ug/lane in A; 5 mg in B) from HeLa cells. Antibody: Affinity purified rabbit anti-CDK7 antibody used at 0.1 ug/ml for WB and at 4 ug/5 mg lysate for IP. Detection: Chemiluminescence with an exposure time of 5 minutes in A. Coomassie stain in B. The identity of the band that is CDK7 was verified by mass spectrometry.

Western blot

Detection of Mouse and Rat CDK7 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-CDK7 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of Mouse and Rat CDK7 by Western Blot. Samples: Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-CDK7 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Western blot

Detection of Human CDK7 by Western Blot and Immunoprecipitation. Samples: RIPA lysate (5 and 25 ug/lane in A; 5 mg in B) from HeLa cells. Antibody: Affinity purified rabbit anti-CDK7 antibody used at 0.1 ug/ml for WB and at 4 ug/5 mg lysate for IP. Detection: Chemiluminescence with an exposure time of 5 minutes in A. Coomassie stain in B. The identity of the band that is CDK7 was verified by mass spectrometry.
Detection of Human CDK7 by Western Blot and Immunoprecipitation. Samples: RIPA lysate (5 and 25 ug/lane in A; 5 mg in B) from HeLa cells. Antibody: Affinity purified rabbit anti-CDK7 antibody used at 0.1 ug/ml for WB and at 4 ug/5 mg lysate for IP. Detection: Chemiluminescence with an exposure time of 5 minutes in A. Coomassie stain in B. The identity of the band that is CDK7 was verified by mass spectrometry.

Requested From: United States
Date Requested: 1/19/2019
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