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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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CDK5R1 Antibody (Gln31) LS‑C176814 Best Seller
CDK5R1 antibody LS-C176814 is an unconjugated rabbit polyclonal antibody to CDK5R1 from human, mouse and rat. Validated for IF, IHC and WB.
Catalog
Size
Price
LS-C176814-100
100 µl
$345

Popular CDK5R1 Products

Antibody
Species: Human
Applications: Immunofluorescence, Western blot, ELISA
Antibody
Species: Human
Applications: IHC, Western blot, ELISA
Antibody
Species: Human
Applications: IHC, ELISA
Immunofluorescent analysis of p35 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Species: Human, Mouse, Rat, Bovine, Zebrafish
Applications: ICC, Immunofluorescence, Western blot

Product Description

CDK5R1 antibody LS-C176814 is an unconjugated rabbit polyclonal antibody to CDK5R1 from human, mouse and rat. Validated for IF, IHC and WB.
About CDK5R1
CDK5R1 (p35) is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. Q15078 NM_003885 NP_003876.1

CDK5R1 Antibody, CDK5R Antibody, CDK5P35 Antibody, p35 Antibody, p35nck5a Antibody, p23 Antibody, p25 Antibody, TPKII regulatory subunit Antibody, CDK5 activator 1 Antibody, NCK5A Antibody, Neuronal CDK5 activator Antibody


Specifications

Target
Human CDK5R1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • Immunofluorescence
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CDK5R1 antibody was raised against synthetic peptide around Gln31 of human p35
Epitope
Gln31
Specificity
Human p35
Presentation
PBS, pH 7.2, 0.05% Sodium Azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Western blot

Western blot of p35 (Q31) pAb in extracts from rat brain.
Western blot of p35 (Q31) pAb in extracts from rat brain.

Western blot

Western blot of p35 (Q31) pAb in extracts from rat brain.
Western blot of p35 (Q31) pAb in extracts from rat brain.

Western blot

Western blot of p35 (Q31) pAb in extracts from rat brain.
Western blot of p35 (Q31) pAb in extracts from rat brain.

Western blot

Western blot of p35 (Q31) pAb in extracts from rat brain.
Western blot of p35 (Q31) pAb in extracts from rat brain.

Requested From: United States
Date Requested: 8/16/2018

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