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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CDC2 + CDK2 + CDK3 Antibody (AP) for ICC, WB/Western, IP LS-C506887

CDC2 + CDK2 + CDK3 antibody LS-C506887 is an AP-conjugated rabbit polyclonal antibody to CDC2 + CDK2 + CDK3 from human, mouse and rat. Validated for ICC, IP and WB.
Catalog
Size
Price
LS-C506887-100
100 µl
$800
Description
CDC2 + CDK2 + CDK3 antibody LS-C506887 is an AP-conjugated rabbit polyclonal antibody to CDC2 + CDK2 + CDK3 from human, mouse and rat. Validated for ICC, IP and WB.
Target
Human CDC2 + CDK2 + CDK3
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Alkaline Phosphatase. Also available conjugated with APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • ICC
  • Western blot
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
Immunogen
Synthetic peptide corresponding to aa 42-57, EGVPSTAIREISLLKE-GGC, of human cdc2 kinase. The immunogen is identical in a wide range of species.
Specificity
Recognizes human cdc2 kinase (34kD). Species cross-reactivity: Mouse, rat, sheep, chicken, frog and starfish. Wide species cross-reactivity is expected.
Presentation
PBS, pH 7.2, No preservatives added
Storage
Store at 4°C.
Restrictions
For research use only.

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Immunofluorescent analysis of CDK1/2/3 (pT14) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Requested From: United States
Date Requested: 1/19/2019
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