Human CD99

Goat

Human (tested or 100% immunogen sequence identity)

IgG3 Polyclonal

Unconjugated

Ascites

Unmodified

HPB MLT T cell line.

Recognizes the human CD99R cell surface antigen.

• Western blot (0.1 - 0.2 µg/ml)
• Flow Cytometry
• ELISA (0.5 - 1 µg/ml)

Suitable for use in ELISA, Western Blot, Flow Cytometry. Western Blot: 0.1-0.2 ug/ml with the appropriate secondary reagents to detect mouse CD99-L2. The detection limit for rmCD99-L2 is approximately 2 ng/lane under non-reducing and reducing. conditions. Flow Cytometry: Dilute this antibody to 25 ug/ml and add 10 ul of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200ul, using bEnd.3 cells (Schenkel AR, et al., (2007) Cell Commun. Adhes. 14:227). The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as anti-goat IgG conjugated to a fluorochrome. Between steps, wash cells in Flow Cytometry Staining Buffer. ELISA: 0.5-1 ug/ml with the appropriate secondary reagents to detect mouse CD99-L2. The detection limit for rmCD99-L2 is approximately 0.2 ng/well.

Lyophilized from Sterile Solution PBS, 5% Trehalose

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P14209 NCBI: NM_002414 NP_002405.1