The FN50 antibody has been pre-titrated and tested by flow cytometric analysis of resting and 6-hour TPA-activated human PBMC. This can be used at 20 ul (0.125 ug)/per test. A test is defined as the amount (ug)/test of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
PBS, pH 7.2, 150 mM sodium chloride, 0.09% sodium azide, 0.2% BSA
Store at 4°C. Do not freeze. Protect from light.
This antibody carries the LSBio 100% Guarantee.