CD59 antibody LS-C391304 is an unconjugated mouse monoclonal antibody to human CD59. Validated for Flow, IF and IHC.
(tested or 100% immunogen sequence identity)
Also available Unmodified
CD59 antibody was raised against recombinant Full-length Human Protein.
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. CD59 is a glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein. CD59 attaches to host cells via a glycophosphatidylinositol (GPI) anchor. When complement activation leads to deposition of C5b678 on host cells, it can prevent C9 from polymerizing and forming the complement membrane attack complex. Mutations affecting GPI that reduce expression of CD59 and decay-accelerating factor on red blood cells result in paroxysmal nocturnal hemoglobinuria. Viruses such as HIV, human cytomegalovirus and vaccinia incorporate host cell CD59 into their own viral envelope to prevent lysis by complement. Optimal dilution of the CD59 antibody should be determined by the researcher. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.