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Catalog Number Size Price
LS-C818136-100 100 µl $412 
CD58 Antibody - Immunofluorescence staining of CD58 in Raji cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human CD58 polyclonal antibody (dilution ratio 1:5000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane.
CD58 Antibody - Anti-CD58 rabbit polyclonal antibody at 1:500 dilution. Lane A: Raji Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 72 kDa.
CD58 Antibody - Immunofluorescence staining of CD58 in Raji cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human CD58 polyclonal antibody (dilution ratio 1:5000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane.
CD58 Antibody - Anti-CD58 rabbit polyclonal antibody at 1:500 dilution. Lane A: Raji Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lane C: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 28 kDa. Observed band size: 72 kDa.
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Polyclonal Rabbit anti‑Human CD58 Antibody (Preservative Free, IF, WB) LS‑C818136

Polyclonal Rabbit anti‑Human CD58 Antibody (Preservative Free, IF, WB) LS‑C818136

Antibody:
CD58 Rabbit anti-Human Polyclonal (Preservative Free) Antibody
Application:
ICC, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Preservative Free
Price
Catalog Number
$412
LS-C818136-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CD58 Rabbit anti-Human Polyclonal (Preservative Free) Antibody
Application:
ICC, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Preservative Free

Specifications

Description
CD58 antibody LS-C818136 is an unconjugated rabbit polyclonal antibody to human CD58. Validated for ELISA, ICC, IF and WB.
Target
Human CD58
Synonyms
CD58 | CD58 antigen | AG3 | LFA-3 | LFA3 | Surface glycoprotein LFA-3 | Surface glycoprotein lfa3 | CD58 molecule
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Preservative Free
Immunogen
Recombinant Human LFA-3 / CD58 Protein
Specificity
Human CD58
Applications
  • ICC (1:1500 - 1:50000)
  • Immunofluorescence (1:1500 - 1:50000)
  • Western blot (1:500 - 1:1000)
  • ELISA (1:25000 - 1:50000)
Usage
Applications should be user optimized.
Presentation
0.2 µm filtered solution in PBS
Storage
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD58
P19256 NM_001779 NP_001770.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/27/2024