CD55 antibody LS-C667747 is an unconjugated rabbit polyclonal antibody to human CD55. Validated for ICC, IF and WB.
(tested or 100% immunogen sequence identity)
- Immunofluorescence (1:50 - 1:200)
- Western blot (1:500 - 1:2000)
CD55 antibody was raised against recombinant full length protein of human CD55
Recognizes endogenous levels of CD55 protein.
Western blot analysis of CD55 expression in Hela (A), A549 (B) whole cell lysates. Immunofluorescent analysis of CD55 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
For research use only.