CD55 antibody LS-C45440 is an unconjugated mouse monoclonal antibody to human CD55. Validated for Flow and IP.
(tested or 100% immunogen sequence identity)
Protein G purified
- Flow Cytometry
CD55 antibody was raised against human fibroblast cell line.
Recognizes the CD55 antigen, a 70kD glycoprotein also known as Decay Accelerating Factor (DAF). Distributed on erythrocytes and other circulating blood cells and also on cells in non-hemopoietic tissue particularly epithelium and endothelium. Found specifically at the fetal-maternal interfaces in placenta. CD55 has reduced expression on individuals with paroxysmal nocturnal hemoglobinuria. Has a functional binding affinity to erythrocytes of 8.7 x 107 M-1. The antigen is pronase and trypsin resistant and chymotrypsin sensitive. Recognizes the consensus region 3 of the DAF molecule, which contains the functional site, and the antibody blocks the function of DAF.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul Method sheets are available on request.
0.09% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.