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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD55 Antibody (clone 67) LS‑C11548
Note: This antibody replaces LS-C57446, LS-C11547
CD55 antibody LS-C11548 is an unconjugated mouse monoclonal antibody to human CD55. Validated for Flow and IHC.
100 µg (1 mg/ml)

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Anti-CD55 antibody IHC of human skin, pilosebaceous unit. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1851 concentration 10 ug/ml.
Species: Human, Non-Human Primates
Applications: IHC, IHC - Paraffin, Immunoprecipitation, Flow Cytometry
Species: Human
Applications: IHC, IHC - Frozen, Flow Cytometry
Species: Mouse
Applications: Western blot, Flow Cytometry, ELISA

Product Description

CD55 antibody LS-C11548 is an unconjugated mouse monoclonal antibody to human CD55. Validated for Flow and IHC.


Human CD55
CD55, CD55 antigen, Decay-accelerating factor, TC, CR, CROM, DAF
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [67]
Tissue culture supernatant
  • IHC
  • IHC - Frozen (1:100 - 1:1000)
  • Flow Cytometry (1:10 - 1:25)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
CD55 antibody was raised against k562 cells.
Recognizes the human CD55 cell surface antigen, a GPI linked molecule also known as decay accelerating factor (DAF). CD55 is expressed by a wide range of cell types. Erythrocyte expression is very weak. CD55 is the complement regulatory protein, decay accelerating factor (DAF). Human CD55 is a ~70kD glycoprotein (in erythrocytes) anchored in the membrane by glycosylphosphatidylinositol tail. In other cells the apparent molecular weight is somewhat larger. It has a substantial content of O-glycans, and also on N-glycan. DAF binds to activated C4b or C3b complement fragments on the cell surface, preventing the assembly and accelerating the decay of both classical and alternative pathways. DAF carries the Cromer related blood group antigens. DAF has a wide distribution on cells in non-hemopoietic tissues, particularly epithelium and is specifically found at the fetal-maternal interface in placenta and. Soluble forms of DAF are found, for example, in plasma, saliva and urine. The antigen on erythrocytes is pronase and chymotrypsin sensitive, but resistant to trypsin.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request. We do not recommend the use of this reagent to stain erythrocytes.
PBS, pH 7.4, 0.09% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
About CD55
This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. P08174 NM_000574 NP_000565.1

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Requested From: United States
Date Requested: 10/20/2018

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