CD55 antibody LS-C11548 is an unconjugated mouse monoclonal antibody to human CD55. Validated for Flow and IHC.
(tested or 100% immunogen sequence identity)
Tissue culture supernatant
- IHC - Frozen (1:100 - 1:1000)
- Flow Cytometry (1:10 - 1:25)
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CD55 antibody was raised against k562 cells.
Recognizes the human CD55 cell surface antigen, a GPI linked molecule also known as decay accelerating factor (DAF). CD55 is expressed by a wide range of cell types. Erythrocyte expression is very weak. CD55 is the complement regulatory protein, decay accelerating factor (DAF). Human CD55 is a ~70kD glycoprotein (in erythrocytes) anchored in the membrane by glycosylphosphatidylinositol tail. In other cells the apparent molecular weight is somewhat larger. It has a substantial content of O-glycans, and also on N-glycan. DAF binds to activated C4b or C3b complement fragments on the cell surface, preventing the assembly and accelerating the decay of both classical and alternative pathways. DAF carries the Cromer related blood group antigens. DAF has a wide distribution on cells in non-hemopoietic tissues, particularly epithelium and is specifically found at the fetal-maternal interface in placenta and. Soluble forms of DAF are found, for example, in plasma, saliva and urine. The antigen on erythrocytes is pronase and chymotrypsin sensitive, but resistant to trypsin.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request. We do not recommend the use of this reagent to stain erythrocytes.
PBS, pH 7.4, 0.09% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.