Human CD3E

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

UCHT1

Unconjugated

Protein A purified

Fab

Human thymocytes/Sezary T cells

Antibody UCHT1 recognizes the 20 kd epsilon chain (3) of the CD3 molecule complex (1, 2).

• Western blot
• Flow Cytometry

Five x 105 human HPB-MLT cultured tumor cells were washed and and incubated 45 minutes on ice with 80 ul of anti-CD3 Fab at 5 ug/ml. Cells were washed twice and incubated with 50 ul of 2o reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.64 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Sterile Solution 50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, No preservatives added

Store at 4°C. Do not freeze. Open under aseptic conditions.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

NCBI: NM_000733 NP_000724.1