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Catalog Number Size Price
LS-C818758-100 100 µl $412 
CD225 / IFITM1 Antibody - Immunofluorescence staining of IFITM1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human IFITM1 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.
CD225 / IFITM1 Antibody - Anti-IFITM1 rabbit monoclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 14 kDa. Observed band size: 18 kDa.
CD225 / IFITM1 Antibody - CD225 was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate. 2 uL anti-CD225 rabbit monoclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-CD225 rabbit monoclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 15 kDa. Observed band size: 15 kDa.
CD225 / IFITM1 Antibody - Immunofluorescence staining of IFITM1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human IFITM1 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to Cytoplasm.
CD225 / IFITM1 Antibody - Anti-IFITM1 rabbit monoclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 14 kDa. Observed band size: 18 kDa.
CD225 / IFITM1 Antibody - CD225 was immunoprecipitated using: Lane A: 0.5 mg K562 Whole Cell Lysate. 2 uL anti-CD225 rabbit monoclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-CD225 rabbit monoclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 15 kDa. Observed band size: 15 kDa.
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Monoclonal Rabbit anti‑Human CD225 / IFITM1 Antibody (clone 021, Preservative Free, IF, WB) LS‑C818758

Monoclonal Rabbit anti‑Human CD225 / IFITM1 Antibody (clone 021, Preservative Free, IF, WB) LS‑C818758

Antibody:
CD225 / IFITM1 Rabbit anti-Human Monoclonal (Preservative Free) (021) Antibody
Application:
ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Preservative Free
Price
Catalog Number
$412
LS-C818758-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CD225 / IFITM1 Rabbit anti-Human Monoclonal (Preservative Free) (021) Antibody
Application:
ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Preservative Free

Specifications

Description
IFITM1 antibody LS-C818758 is an unconjugated rabbit monoclonal antibody to human IFITM1 (CD225). Validated for ICC, IF, IP and WB.
Target
Human CD225 / IFITM1
Synonyms
IFITM1 | 9-27 | CD225 antigen | Dispanin subfamily A member 2a | DSPA2a | IFI17 | LEU13 | CD225 | Interferon-induced protein 17 | Leu-13 antigen
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal
Clone
021
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Preservative Free
Immunogen
A synthetic peptide corresponding to the center region of the Human IFITM1
Specificity
Human IFITM1/CD225
Applications
  • ICC (1:20 - 1:100)
  • Immunofluorescence (1:20 - 1:100)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation
Usage
Applications should be user optimized.
Presentation
0.2 µm filtered solution in PBS
Storage
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD225 / IFITM1
P13164 NM_003641 NP_003632.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/30/2024