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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD20 Antibody (aa284-297) IHC-plus™ LS-B11144

Note: This antibody replaces LS-C20141
Anti-CD20 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Goat Anti-CD20 / MS4A1 (C Terminus) Antibody (0.05µg/ml) staining of Human Lymph Node lysate (35µg protein in RIPA buffer). Detected by chemiluminescencence.
Staining (4µg/ml) of Daudi lysate (RIPA buffer, 20µg total protein per lane). Primary incubated for 12 hour. Detected using chemiluminescence.
Anti-CD20 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Goat Anti-CD20 / MS4A1 (C Terminus) Antibody (0.05µg/ml) staining of Human Lymph Node lysate (35µg protein in RIPA buffer). Detected by chemiluminescencence.
Staining (4µg/ml) of Daudi lysate (RIPA buffer, 20µg total protein per lane). Primary incubated for 12 hour. Detected using chemiluminescence.
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CD20 Antibody (aa284-297) IHC-plus™ LS-B11144

Note: This antibody replaces LS-C20141
Antibody:
Goat Polyclonal to Human CD20
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B11144-50
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human CD20
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Format:
Unconjugated, Unmodified

Specifications

Description
CD20 antibody LS-B11144 is an unconjugated goat polyclonal antibody to CD20. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.

Target
Human CD20
Host
Goat
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2 - 2.5 µg/ml)
  • Western blot (0.03 - 0.1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CD20 antibody was raised against peptide with sequence CQDQESSPIENDSSP, from the C Terminus of the protein sequence according to NP_068769.2NP_690605.1.
Blocking Peptide
LS-E46587 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B11144. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa284-297
Specificity
Human CD20 / MS4A1
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD20
P11836 NM_021950 NP_690605.1

Validation

Anti-CD20 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
IHC Validated
Anti-CD20 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-CD20 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC Validated
Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

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Featured Products

Product
Species: Human
Applications: ICC, Western blot, Flow Cytometry, ELISA
Reactivity: Human
Range: 78.125-5000 pg/ml
Reactivity: Chicken
Range: 0.16-10 ng/ml
Anti-CD20 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay

Requested From: United States
Date Requested: 7/19/2019
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