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Catalog Number Size Price
LS-C758310-100 100 µg (0.5 mg/ml) $454 
CD144 / CDH5 / VE Cadherin Antibody - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Polyclonal Goat anti‑Human CD144 / CDH5 / VE Cadherin Antibody (Amino Acids 537‑549) LS‑C758310

Polyclonal Goat anti‑Human CD144 / CDH5 / VE Cadherin Antibody (Amino Acids 537‑549) LS‑C758310

Antibody:
CD144 / CDH5 / VE Cadherin Goat anti-Human Polyclonal (Amino Acids 537-549) Antibody
Application:
Flo, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$454
LS-C758310-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CD144 / CDH5 / VE Cadherin Goat anti-Human Polyclonal (Amino Acids 537-549) Antibody
Application:
Flo, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
VE Cadherin antibody LS-C758310 is an unconjugated goat polyclonal antibody to human VE Cadherin (CD144 / CDH5) (Amino Acids 537-549). Validated for Flow and Peptide-ELISA.
Target
Human CD144 / CDH5 / VE Cadherin
Synonyms
CDH5 | 7B4 | 7B4 antigen | CD144 | Cadherin-5 | Endothelial-specific cadherin | Vascular endothelial cadherin | VE-cadherin | VEC | CD144 antigen | VE Cadherin
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-TVKYGQFDREHTK, from the internal region of the protein sequence according to NP_001786.2.
Epitope
Amino Acids 537-549
Applications
  • Flow Cytometry (10 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Preliminary experiments gave an approx 100kDa band in lysates of cell line HeLa and approx. 110kDa in A549 at 1-2µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observed, given the calculated size of 87.5kDa according to NP_001786.2. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD144 / CDH5 / VE Cadherin
P33151 NM_001795 NP_001786.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/27/2024