Target
Human CD144 / CDH5 / VE Cadherin
Synonyms
CDH5 | 7B4 | 7B4 antigen | CD144 | Cadherin-5 | Endothelial-specific cadherin | Vascular endothelial cadherin | VE-cadherin | VEC | CD144 antigen | VE Cadherin
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-TVKYGQFDREHTK, from the internal region of the protein sequence according to NP_001786.2.
Epitope
Amino Acids 537-549
Applications
- Flow Cytometry (10 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Preliminary experiments gave an approx 100kDa band in lysates of cell line HeLa and approx. 110kDa in A549 at 1-2µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observed, given the calculated size of 87.5kDa according to NP_001786.2. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CD144 / CDH5 / VE Cadherin