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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD121b / IL1R2 Antibody (aa172‑398) LS‑C186020
IL1R2 antibody LS-C186020 is an unconjugated rabbit polyclonal antibody to human IL1R2 (CD121b). Validated for ICC, IHC and WB.
Catalog
Size
Price
LS-C186020-100
100 µl (1 mg/ml)
$335

Popular CD121b / IL1R2 Products

Antibody
Species: Human
Applications: Flow Cytometry
Anti-IL1R2 antibody IHC of human small intestine, Peyer's patch. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Antibody
Species: Human
Applications: Western blot, Flow Cytometry
Antibody
Species: Human
Applications: ICC, Western blot, Flow Cytometry, ELISA
Western blot. All lanes: Interleukin-1 receptor type 2 antibody at 3 ug/ml+mouse kidney tissue. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 45 kDa. Observed band size: 45 kDa.
Species: Human, Mouse
Applications: Western blot, ELISA

Product Description

IL1R2 antibody LS-C186020 is an unconjugated rabbit polyclonal antibody to human IL1R2 (CD121b). Validated for ICC, IHC and WB.

Specifications

Target
Human CD121b / IL1R2
Synonyms
IL1R2, CD121b, CD121b antigen, CDw121b, IL-1RT-2, IL1R2c, Interleukin-1 receptor beta, Interleukin-1 receptor type II, IL-1R-beta, Antigen CDw121b, IL-1 type II receptor, IL-1R-2, IL-1RT2, IL1RB, Interleukin-1 receptor type 2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:1000)
  • ICC (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CD121b / IL1R2 antibody was raised against recombinant fragment corresponding to a region within amino acids 172 and 398 of IL1 Receptor 2 (SwissProt P27930). Percent identity by BLAST analysis: Human (100%); Monkey (94%).
Epitope
aa172-398
Specificity
Human CD121b / IL1R2
Usage
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
Presentation
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Restrictions
For research use only.
About CD121b / IL1R2
Non-signaling receptor for IL1A, IL1B and IL1RN. Reduces IL1B activities. Serves as a decoy receptor by competetive binding to IL1B and preventing its binding to IL1R1. Also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. P27930 NM_004633 NP_004624.1


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Images

Immunohistochemistry

IHC of paraffin-embedded SW480 xenograft using IL1R2 antibody at 1:100 dilution.
IHC of paraffin-embedded SW480 xenograft using IL1R2 antibody at 1:100 dilution.

Immunocytochemistry

Immunofluorescence of methanol-fixed A549 using IL1 Receptor 2 antibody at 1:500 dilution.
Immunofluorescence of methanol-fixed A549 using IL1 Receptor 2 antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate). A:293T. 10% SDS PAGE. CD121b / IL1R2 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate). A:293T. 10% SDS PAGE. CD121b / IL1R2 antibody diluted at 1:1000

Immunohistochemistry

IHC of paraffin-embedded SW480 xenograft using IL1R2 antibody at 1:100 dilution.
IHC of paraffin-embedded SW480 xenograft using IL1R2 antibody at 1:100 dilution.

Immunocytochemistry

Immunofluorescence of methanol-fixed A549 using IL1 Receptor 2 antibody at 1:500 dilution.
Immunofluorescence of methanol-fixed A549 using IL1 Receptor 2 antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate). A:293T. 10% SDS PAGE. CD121b / IL1R2 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate). A:293T. 10% SDS PAGE. CD121b / IL1R2 antibody diluted at 1:1000

Immunohistochemistry

IHC of paraffin-embedded SW480 xenograft using IL1R2 antibody at 1:100 dilution.
IHC of paraffin-embedded SW480 xenograft using IL1R2 antibody at 1:100 dilution.

Immunocytochemistry

Immunofluorescence of methanol-fixed A549 using IL1 Receptor 2 antibody at 1:500 dilution.
Immunofluorescence of methanol-fixed A549 using IL1 Receptor 2 antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate). A:293T. 10% SDS PAGE. CD121b / IL1R2 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate). A:293T. 10% SDS PAGE. CD121b / IL1R2 antibody diluted at 1:1000

Immunohistochemistry

IHC of paraffin-embedded SW480 xenograft using IL1R2 antibody at 1:100 dilution.
IHC of paraffin-embedded SW480 xenograft using IL1R2 antibody at 1:100 dilution.

Immunocytochemistry

Immunofluorescence of methanol-fixed A549 using IL1 Receptor 2 antibody at 1:500 dilution.
Immunofluorescence of methanol-fixed A549 using IL1 Receptor 2 antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate). A:293T. 10% SDS PAGE. CD121b / IL1R2 antibody diluted at 1:1000
Sample (30 ug of whole cell lysate). A:293T. 10% SDS PAGE. CD121b / IL1R2 antibody diluted at 1:1000

Requested From: United States
Date Requested: 11/15/2018

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