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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CD118 / LIF Receptor Alpha Antibody (clone 4A10) LS‑C197615
LIF Receptor Alpha antibody LS-C197615 is an unconjugated mouse monoclonal antibody to human LIF Receptor Alpha (CD118). Validated for ELISA.
Catalog
Size
Price
LS-C197615-100
100 µg
$355
Description
LIF Receptor Alpha antibody LS-C197615 is an unconjugated mouse monoclonal antibody to human LIF Receptor Alpha (CD118). Validated for ELISA.
Target
Human CD118 / LIF Receptor Alpha
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [4A10]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • ELISA
Immunogen
LIFR (NP_002301, aa45-154 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human CD118 / LIFR
Usage
Sandwich ELISA: Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
About CD118 / LIF Receptor Alpha
P42702 NM_002310 NP_002301.1

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Western blot of recombinant CD118 / LIFR.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Immunohistochemistry image at a dilution of 1:600 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 °C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged LIFR is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged LIFR is approximately 0.03 ng/ml as a capture antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged LIFR is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged LIFR is approximately 0.03 ng/ml as a capture antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged LIFR is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged LIFR is approximately 0.03 ng/ml as a capture antibody.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged LIFR is approximately 0.03 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged LIFR is approximately 0.03 ng/ml as a capture antibody.

Requested From: United States
Date Requested: 11/20/2018
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