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Antibodies > CCT5 / TCP1 Epsilon > LS-C661889
 
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Catalog Number Size Price
LS-C661889-10 10 µg $318 
LS-C661889-100 100 µg $470 
CCT5 / TCP1 Epsilon Antibody - IHC analysis of CCT5 using anti-CCT5 antibody. CCT5 was detected in paraffin-embedded section of mouse ovary tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CCT5 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CCT5 / TCP1 Epsilon Antibody - Immunohistochemistry - Anti-TCP1 epsilon Picoband Antibody
CCT5 / TCP1 Epsilon Antibody - TCP1 epsilon was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti- TCP1 epsilon Antigen Affinity purified polyclonal antibody
CCT5 / TCP1 Epsilon Antibody - TCP1 epsilon was detected in paraffin-embedded sections of rat ovary tissues using rabbit anti- TCP1 epsilon Antigen Affinity purified polyclonal antibody
CCT5 / TCP1 Epsilon Antibody - IF analysis of CCT5 using anti-CCT5 antibody CCT5 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-CCT5 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CCT5 / TCP1 Epsilon Antibody - Western blot analysis of CCT5 using anti-CCT5 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCT5 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CCT5 at approximately 60KD. The expected band size for CCT5 is at 60KD.
CCT5 / TCP1 Epsilon Antibody - Flow Cytometry analysis of A431 cells using anti-CCT5 antibody. Overlay histogram showing A431 cells stained with anti-CCT5 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCT5 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
CCT5 / TCP1 Epsilon Antibody - IHC analysis of CCT5 using anti-CCT5 antibody. CCT5 was detected in paraffin-embedded section of mouse ovary tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CCT5 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
CCT5 / TCP1 Epsilon Antibody - Immunohistochemistry - Anti-TCP1 epsilon Picoband Antibody
CCT5 / TCP1 Epsilon Antibody - TCP1 epsilon was detected in paraffin-embedded sections of human intestinal cancer tissues using rabbit anti- TCP1 epsilon Antigen Affinity purified polyclonal antibody
CCT5 / TCP1 Epsilon Antibody - TCP1 epsilon was detected in paraffin-embedded sections of rat ovary tissues using rabbit anti- TCP1 epsilon Antigen Affinity purified polyclonal antibody
CCT5 / TCP1 Epsilon Antibody - IF analysis of CCT5 using anti-CCT5 antibody CCT5 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-CCT5 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CCT5 / TCP1 Epsilon Antibody - Western blot analysis of CCT5 using anti-CCT5 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: HELA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCT5 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CCT5 at approximately 60KD. The expected band size for CCT5 is at 60KD.
CCT5 / TCP1 Epsilon Antibody - Flow Cytometry analysis of A431 cells using anti-CCT5 antibody. Overlay histogram showing A431 cells stained with anti-CCT5 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCT5 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human CCT5 / TCP1 Epsilon Antibody (IHC, WB) LS‑C661889

Polyclonal Rabbit anti‑Human CCT5 / TCP1 Epsilon Antibody (IHC, WB) LS‑C661889

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Antibody:
CCT5 / TCP1 Epsilon Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C661889-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CCT5 / TCP1 Epsilon Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
TCP1 Epsilon antibody LS-C661889 is an unconjugated rabbit polyclonal antibody to human TCP1 Epsilon (CCT5). Validated for ICC, IHC and WB.
Target
Human CCT5 / TCP1 Epsilon
Synonyms
CCT5 | KIAA0098 | CCT-epsilon | CCTE | TCP-1-epsilon | TCP1 Epsilon
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived human TCP1 epsilon recombinant protein (Position: L443-K535). Human TCP1 epsilon shares 95.7% amino acid (aa) sequence identity with both mouse and rat TCP1 epsilon.
Applications
  • IHC
  • IHC - Paraffin
  • ICC
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CCT5 / TCP1 Epsilon
P48643 D43950 BAA07894.2

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See all CCT5 / TCP1 Epsilon Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/29/2024
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