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Catalog Number Size Price
LS-C745372-25 25 µl (1 mg/ml) $275 
CCND3 / Cyclin D3 Antibody - Immunohistochemistry with anti-Cyclin D3 antibody showing Cyclin D3 staining in nucleus of lymphocytes in human spleen at 20x and 40x (B & C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-mouse Cyclin D3 antibody at 4.0 µg/ml for 60 minutes. The reaction was developed using MACH 4 universal AP polymer detection system and visualized with WARP RED.
CCND3 / Cyclin D3 Antibody - Immunoprecipitation of rabbit Anti-Cyclin D3. Lane 1: 5µg anti-cyclin D3 antibody/IP. Primary Antibody: Cyclin D3 Mouse mAb. Lane 2: 5µg anti-cyclin D3 antibody/IP. Primary Antibody: Cyclin D2 rabbit pAB. Secondary Antibody: TrueBlot IgG IP beads. Expect: ~32kDa to D3 in lane 1. No reactivity to D2 in lane 2.
CCND3 / Cyclin D3 Antibody - Flow Cytometry of rabbit anti-Cyclin D3 antibody. Cells: mouse B cells+, 14 days post immunization (NP-CGG). Anti-Cyclin D3-RPE antibody at 1:500 for 20 min at 4°C.
CCND3 / Cyclin D3 Antibody - ELISA results of purified Rabbit Anti-Cyclin D3 Antibody tested against BSA-conjugated peptide of cyclin D3 peptide. Each well was coated in duplicate with 0.1µg of conjugate (red line). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer
CCND3 / Cyclin D3 Antibody - Immunohistochemistry with anti-Cyclin D3 antibody showing Cyclin D3 staining in nucleus of lymphocytes in human spleen at 20x and 40x (B & C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-mouse Cyclin D3 antibody at 4.0 µg/ml for 60 minutes. The reaction was developed using MACH 4 universal AP polymer detection system and visualized with WARP RED.
CCND3 / Cyclin D3 Antibody - Immunoprecipitation of rabbit Anti-Cyclin D3. Lane 1: 5µg anti-cyclin D3 antibody/IP. Primary Antibody: Cyclin D3 Mouse mAb. Lane 2: 5µg anti-cyclin D3 antibody/IP. Primary Antibody: Cyclin D2 rabbit pAB. Secondary Antibody: TrueBlot IgG IP beads. Expect: ~32kDa to D3 in lane 1. No reactivity to D2 in lane 2.
CCND3 / Cyclin D3 Antibody - Flow Cytometry of rabbit anti-Cyclin D3 antibody. Cells: mouse B cells+, 14 days post immunization (NP-CGG). Anti-Cyclin D3-RPE antibody at 1:500 for 20 min at 4°C.
CCND3 / Cyclin D3 Antibody - ELISA results of purified Rabbit Anti-Cyclin D3 Antibody tested against BSA-conjugated peptide of cyclin D3 peptide. Each well was coated in duplicate with 0.1µg of conjugate (red line). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer
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Polyclonal Rabbit anti‑Mouse CCND3 / Cyclin D3 Antibody (C‑Terminus, IHC, WB) LS‑C745372

Polyclonal Rabbit anti‑Mouse CCND3 / Cyclin D3 Antibody (C‑Terminus, IHC, WB) LS‑C745372

Antibody:
CCND3 / Cyclin D3 Rabbit anti-Mouse Polyclonal (C-Terminus) Antibody
Application:
IHC, WB, IP, Flo, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745372-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
CCND3 / Cyclin D3 Rabbit anti-Mouse Polyclonal (C-Terminus) Antibody
Application:
IHC, WB, IP, Flo, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Cyclin D3 antibody LS-C745372 is an unconjugated rabbit polyclonal antibody to mouse Cyclin D3 (CCND3) (C-Terminus). Validated for ELISA, Flow, IHC, IP and WB.
Target
Mouse CCND3 / Cyclin D3
Synonyms
CCND3 | D3-type cyclin | G1/S-specific cyclin D3 | G1/S-specific cyclin-D3 | Cyclin D3
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Predicted
Human, Bovine (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal domain of mouse Cyclin D3 protein.
Epitope
C-Terminus
Applications
  • IHC (4 µg/ml)
  • Western blot (1 µg/ml)
  • Immunoprecipitation (5 µg/ml)
  • Flow Cytometry (4 µg/ml)
  • ELISA (1:1000 - 1:5000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CCND3 / Cyclin D3
P30281 NM_001760 NP_001751.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/14/2021