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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C154130-100 100 µg (1 mg/ml) $425 
CCL3 / MIP-1-Alpha Antibody - Anti-Mouse MIP-1a Antibody - Western Blot. Anti-mouse MIP-1a in western blot shows detection of recombinant mouse MIP-1a/Ccl3 raised in E. coli. Recombinant truncated (0.1 ug, 7.8 kD) protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with Anti-Mouse MIP-1a/Ccl3. After washing, membrane was probed with secondary antibody Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody ( diluted 1:20000 in blocking buffer (MB-070) for 30 min. at RT. Data was collected using Bio-Rad VersaDoc 4000 MP imaging system.

Polyclonal CCL3 / MIP‑1‑Alpha Antibody (WB) LS‑C154130

Polyclonal CCL3 / MIP‑1‑Alpha Antibody (WB) LS‑C154130

Antibody:
Rabbit Polyclonal to Mouse CCL3 / MIP-1-Alpha
Application:
WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C154130-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Mouse CCL3 / MIP-1-Alpha
Application:
WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
MIP-1-Alpha antibody LS-C154130 is an unconjugated rabbit polyclonal antibody to mouse MIP-1-Alpha (CCL3 ). Validated for ELISA and WB.
Target
Mouse CCL3 / MIP-1-Alpha
Synonyms
CCL3 | C-C motif chemokine 3 | G0S19-1 | Mip1alpha | SCYA3 | SIS-beta | Chemokine (C-C motif) ligand 3 | LD78ALPHA | MIP-1-alpha | MIP1A | PAT 464.1 | Small-inducible cytokine A3
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Unmodified
Applications
  • Western blot (1:1000)
  • ELISA (1:10000)
Immunogen
This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with recombinant truncated mouse MIP-1a protein produced in E.coli.
Specificity
This antibody is specific for mouse MIP-1a protein. Cross-reactivity with MIP-1a from other sources has not been determined.
Usage
This purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 7-8 kD in size corresponding to the mature 92 amino acid mouse MIP-1a/Ccl3 protein by western blotting in appropriate cell lysate or extract.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CCL3 / MIP-1-Alpha

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Requested From: United States
Date Requested: 2/19/2020