Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C756602-10 10 µg $318 
LS-C756602-100 100 µg $470 
CCL16 / LEC Antibody - Western blot analysis of CCL16 using anti-CCL16 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL16 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CCL16 at approximately 18KD. The expected band size for CCL16 is at 14KD.

Polyclonal Rabbit anti‑Human CCL16 / LEC Antibody (WB) LS‑C756602

Polyclonal Rabbit anti‑Human CCL16 / LEC Antibody (WB) LS‑C756602

Antibody:
CCL16 / LEC Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C756602-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CCL16 / LEC Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
LEC antibody LS-C756602 is an unconjugated rabbit polyclonal antibody to human LEC (CCL16). Validated for ELISA and WB.
Target
Human CCL16 / LEC
Synonyms
CCL16 | Cc chemokine lcc-1 | CKb12 | Chemokine hcc-4 | Chemokine LEC | Chemokine lmc | ILINCK | Liver CC chemokine-1 | Monotactin-1 | NCC-4 | LCC-1 | LEC | Small-inducible cytokine A16 | Liver-expressed chemokine | LMC | New CC chemokine 4 | C-C motif chemokine 16 | Chemokine CC-4 | Chemokine ncc-4 | HCC-4 | IL-10-inducible chemokine | Mtn-1 | NCC4 | SCYA16 | SCYL4
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived human CCL16 recombinant protein (Position: Q24-Q120).
Specificity
No cross reactivity with other proteins.
Applications
  • Western blot (0.1 - 0.5 µg/ml)
  • ELISA (1 - 5 µg/ml)
Usage
Applications should be user optimized.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CCL16 / LEC
O15467 NM_004590 NP_004581.1

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/27/2024