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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CAPG Antibody (clone AT1D10) LS-C755414

CAPG antibody LS-C755414 is an unconjugated mouse monoclonal antibody to human CAPG. Validated for ELISA, ICC and WB.
Catalog
Size
Price
LS-C755414-50
50 µl (1 mg/ml)
$275
LS-C755414-100
100 µl (1 mg/ml)
$345
Description
CAPG antibody LS-C755414 is an unconjugated mouse monoclonal antibody to human CAPG. Validated for ELISA, ICC and WB.
Target
Human CAPG
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b,k Monoclonal
Clone
AT1D10
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Applications
  • ICC (1:100)
  • Western blot (1:1000)
  • ELISA
Immunogen
CAPG antibody was raised against recombinant human CAPG (1-348aa) purified from E. coli
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 10% Glycerol
Restrictions
For research use only.
About CAPG
P40121 NM_001747 NP_001738.2

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Images

Immunofluorescence

ICC/IF analysis of CAPG in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CAPG antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
ICC/IF analysis of CAPG in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CAPG antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Western blot

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CAPG antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: NIH3T3 cell lysate Lane 3.: Mouse spleen tissue lysate Lane 4.: THP-1 cell lysate Lane 5.: Raw 264.7 cell lysate
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CAPG antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: NIH3T3 cell lysate Lane 3.: Mouse spleen tissue lysate Lane 4.: THP-1 cell lysate Lane 5.: Raw 264.7 cell lysate

Immunofluorescence

ICC/IF analysis of CAPG in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CAPG antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
ICC/IF analysis of CAPG in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CAPG antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Western blot

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CAPG antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: NIH3T3 cell lysate Lane 3.: Mouse spleen tissue lysate Lane 4.: THP-1 cell lysate Lane 5.: Raw 264.7 cell lysate
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CAPG antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: NIH3T3 cell lysate Lane 3.: Mouse spleen tissue lysate Lane 4.: THP-1 cell lysate Lane 5.: Raw 264.7 cell lysate

Immunofluorescence

ICC/IF analysis of CAPG in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CAPG antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
ICC/IF analysis of CAPG in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CAPG antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Western blot

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CAPG antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: NIH3T3 cell lysate Lane 3.: Mouse spleen tissue lysate Lane 4.: THP-1 cell lysate Lane 5.: Raw 264.7 cell lysate
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CAPG antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: NIH3T3 cell lysate Lane 3.: Mouse spleen tissue lysate Lane 4.: THP-1 cell lysate Lane 5.: Raw 264.7 cell lysate

Immunofluorescence

ICC/IF analysis of CAPG in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CAPG antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
ICC/IF analysis of CAPG in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CAPG antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Western blot

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CAPG antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: NIH3T3 cell lysate Lane 3.: Mouse spleen tissue lysate Lane 4.: THP-1 cell lysate Lane 5.: Raw 264.7 cell lysate
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CAPG antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: NIH3T3 cell lysate Lane 3.: Mouse spleen tissue lysate Lane 4.: THP-1 cell lysate Lane 5.: Raw 264.7 cell lysate

Requested From: United States
Date Requested: 3/23/2019
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