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Antibodies > CALD1 / Caldesmon > LS-C801160
 
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Catalog Number Size Price
LS-C801160-100 100 µl (1 mg/ml) $379 
LS-C801160-200 200 µl (1 mg/ml) $421 
CALD1 / Caldesmon Antibody - 1:200 staining human lymph nodes tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
CALD1 / Caldesmon Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
CALD1 / Caldesmon Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
CALD1 / Caldesmon Antibody - Western blot analysis of Caldesmon phosphorylation expression in EGF treated HeLa whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
CALD1 / Caldesmon Antibody - Western blot analysis of Phospho-Caldesmon (Ser789) expression in various lysates
CALD1 / Caldesmon Antibody - 1:200 staining human lymph nodes tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
CALD1 / Caldesmon Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
CALD1 / Caldesmon Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
CALD1 / Caldesmon Antibody - Western blot analysis of Caldesmon phosphorylation expression in EGF treated HeLa whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
CALD1 / Caldesmon Antibody - Western blot analysis of Phospho-Caldesmon (Ser789) expression in various lysates
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Polyclonal Rabbit anti‑Human CALD1 / Caldesmon Antibody (phospho‑Ser789, IHC, IF, WB) LS‑C801160

Polyclonal Rabbit anti‑Human CALD1 / Caldesmon Antibody (phospho‑Ser789, IHC, IF, WB) LS‑C801160

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Antibody:
CALD1 / Caldesmon Rabbit anti-Human Polyclonal (pSer789) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801160-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
CALD1 / Caldesmon Rabbit anti-Human Polyclonal (pSer789) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Caldesmon antibody LS-C801160 is an unconjugated rabbit polyclonal antibody to Caldesmon (CALD1) (pSer789) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human CALD1 / Caldesmon
Synonyms
CALD1 | Caldesmon | Caldesmon 1 | HCAD | LCAD | H-CAD | NAG22 | CDM | L-CAD
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human Caldesmon around the phosphorylation site of Serine 789.
Epitope
pSer789
Specificity
Phospho-Caldesmon (Ser789) Antibody detects endogenous levels of Caldesmon only when phosphorylated at Serine 789.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CALD1 / Caldesmon
Q05682 NM_004342 NP_004333.1

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Featured Products

CALD1 / Caldesmon Antibody - 293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
CALD1 / Caldesmon Antibody (aa578‑627) LS‑C80522
Species: Human
Applications: IHC, Western blot
CALD1 / Caldesmon Antibody - CALD1 / Caldesmon antibody Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
CALD1 / Caldesmon Antibody (aa467‑516) LS‑C110713
Species: Human
Applications: Western blot
CALD1 / Caldesmon ELISA Kit
Human CALD1 / Caldesmon (Sandwich ELISA) ELISA Kit - LS-F9214
Reactivity: Human
Range: 0.781-50 ng/ml
c-CBL Antibody - Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
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Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry, ELISA
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Reactivity: Human
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Tag: Myc-DDK (Flag)
See all CALD1 / Caldesmon Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/27/2024
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