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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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C/EBP Epsilon / CEBPE Antibody (clone 7A4) LS‑C196895
CEBPE antibody LS-C196895 is an unconjugated mouse monoclonal antibody to human CEBPE (C / EBP Epsilon). Validated for ELISA, RNAi and WB.
Catalog
Size
Price
LS-C196895-100
100 µg
$355

Popular C/EBP Epsilon / CEBPE Products

Anti-C/EBP Epsilon / CEBPE antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B9957 dilution 1:100.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Western blot of HUVEC cell lysate using C/EBP-epsilon Antibody
Species: Human
Applications: IHC, ICC, Immunofluorescence, Western blot
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using C/EBP-epsilon Antibody. The picture on the right is blocked with the synthesized peptide.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Immunohistochemistry analysis of paraffin-embedded human brain, using C/EBP-epsilon (Phospho-Thr74) Antibody. The picture on the right is blocked with the phospho peptide.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, ELISA

Product Description

CEBPE antibody LS-C196895 is an unconjugated mouse monoclonal antibody to human CEBPE (C / EBP Epsilon). Validated for ELISA, RNAi and WB.

Specifications

Target
Human C/EBP Epsilon / CEBPE
Synonyms
CEBPE, CRP1, C/EBP epsilon, C/EBP-epsilon
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [7A4]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
  • RNA interference
Immunogen
CEBPE (AAH35797.2, aa1-281 full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human C/EBP Epsilon / CEBPE
Usage
RNAi Knockdown: Antibody validated. Western Blot: Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
About C/EBP Epsilon / CEBPE
C/EBP Epsilon / CEBPE is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related protein CEBP-delta. The encoded protein may be essential for terminal differentiation and functional maturation of committed granulocyte progenitor cells. Mutations in this gene have been associated with Specific Granule Deficiency, a rare congenital disorder. Q15744 NM_001805 NP_001796.2


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Images

Western blot

Western Blot analysis of CEBPE expression in transfected 293T cell line by CEBPE monoclonal antibody (M01), clone 7A4.Lane 1: CEBPE transfected lysate(30.6 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of CEBPE expression in transfected 293T cell line by CEBPE monoclonal antibody (M01), clone 7A4.Lane 1: CEBPE transfected lysate(30.6 KDa).Lane 2: Non-transfected lysate.

RNA interference

Western blot analysis of CEBPE over-expressed 293 cell line, cotransfected with CEBPE Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CEBPE monoclonal antibody (M01), clone 7A4 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western blot analysis of CEBPE over-expressed 293 cell line, cotransfected with CEBPE Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CEBPE monoclonal antibody (M01), clone 7A4 . GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western blot

Western Blot analysis of CEBPE expression in transfected 293T cell line by CEBPE monoclonal antibody (M01), clone 7A4.Lane 1: CEBPE transfected lysate(30.6 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of CEBPE expression in transfected 293T cell line by CEBPE monoclonal antibody (M01), clone 7A4.Lane 1: CEBPE transfected lysate(30.6 KDa).Lane 2: Non-transfected lysate.

RNA interference

Western blot analysis of CEBPE over-expressed 293 cell line, cotransfected with CEBPE Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CEBPE monoclonal antibody (M01), clone 7A4 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western blot analysis of CEBPE over-expressed 293 cell line, cotransfected with CEBPE Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CEBPE monoclonal antibody (M01), clone 7A4 . GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western blot

Western Blot analysis of CEBPE expression in transfected 293T cell line by CEBPE monoclonal antibody (M01), clone 7A4.Lane 1: CEBPE transfected lysate(30.6 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of CEBPE expression in transfected 293T cell line by CEBPE monoclonal antibody (M01), clone 7A4.Lane 1: CEBPE transfected lysate(30.6 KDa).Lane 2: Non-transfected lysate.

RNA interference

Western blot analysis of CEBPE over-expressed 293 cell line, cotransfected with CEBPE Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CEBPE monoclonal antibody (M01), clone 7A4 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western blot analysis of CEBPE over-expressed 293 cell line, cotransfected with CEBPE Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CEBPE monoclonal antibody (M01), clone 7A4 . GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western blot

Western Blot analysis of CEBPE expression in transfected 293T cell line by CEBPE monoclonal antibody (M01), clone 7A4.Lane 1: CEBPE transfected lysate(30.6 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of CEBPE expression in transfected 293T cell line by CEBPE monoclonal antibody (M01), clone 7A4.Lane 1: CEBPE transfected lysate(30.6 KDa).Lane 2: Non-transfected lysate.

RNA interference

Western blot analysis of CEBPE over-expressed 293 cell line, cotransfected with CEBPE Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CEBPE monoclonal antibody (M01), clone 7A4 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western blot analysis of CEBPE over-expressed 293 cell line, cotransfected with CEBPE Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CEBPE monoclonal antibody (M01), clone 7A4 . GAPDH ( 36.1 kDa ) used as specificity and loading control.

Requested From: United States
Date Requested: 10/18/2018

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