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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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C/EBP Delta / CEBPD Antibody LS‑C750229
CEBPD antibody LS-C750229 is an unconjugated rabbit polyclonal antibody to CEBPD (C / EBP Delta) from human and mouse. Validated for WB.
Catalog
Size
Price
LS-C750229-50
50 µl
$235
Description
CEBPD antibody LS-C750229 is an unconjugated rabbit polyclonal antibody to CEBPD (C / EBP Delta) from human and mouse. Validated for WB.
Target
Human C/EBP Delta / CEBPD
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Western blot (1:200 - 1:2000)
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-269 of human CEBPD (NP_005186.2). MSAALFSLDGPARGAPWPAEPAPFYEPGRAGKPGRGAEPGALGEPGAAAPAMYDDESAIDFSAYIDSMAAVPTLELCHDELFADLFNSNHKAGGAGPLELLPGGPARPLGPGPAAPRLLKREPDWGDGDAPGSLLPAQVAACAQTVVSLAAAGQPTPPTSPEPPRSSPRQTPAPGPAREKSAGKRGPDRGSPEYRQRRERNNIAVRKSRDKAKRRNQEMQQKLVELSAENEKLHQRVEQLTRDLAGLRQFFKQLPSPPFLPAAGTADCR
Usage
The predicted MW is 28kDa, while the observed MW by Western blot was 28kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About C/EBP Delta / CEBPD
P49716 NM_005195 NP_005186.2

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Western Blot of Anti-C/EBPd Antibody Lane 1: untreated wild-type (WT) cells Lane 2: WT cells treated 3 h with LPS Lane 3: C/EBPd-knock-out cells treated 3H with LPS Load: 35 µg per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4°C Blocking: buffer TBST ECL was used for detection of endogenous protein in whole cell extracts from mouse primary macrophage cells. Predicted/Observed size: 28.8 kDa, ~35 kDa
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Images

Western blot

Western blot analysis of extracts of mouse lung, using CEBPD antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90S.
Western blot analysis of extracts of mouse lung, using CEBPD antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90S.

Western blot

Western blot analysis of extracts of mouse lung, using CEBPD antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90S.
Western blot analysis of extracts of mouse lung, using CEBPD antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90S.

Western blot

Western blot analysis of extracts of mouse lung, using CEBPD antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90S.
Western blot analysis of extracts of mouse lung, using CEBPD antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90S.

Western blot

Western blot analysis of extracts of mouse lung, using CEBPD antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90S.
Western blot analysis of extracts of mouse lung, using CEBPD antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90S.

Requested From: United States
Date Requested: 11/17/2018
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