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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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C/EBP Delta / CEBPD Antibody (Biotin) LS‑C687122
CEBPD antibody LS-C687122 is a biotin-conjugated rabbit polyclonal antibody to CEBPD (C / EBP Delta) from mouse and rat. Validated for IHC and WB.
Catalog
Size
Price
LS-C687122-20
20 µl
$205
LS-C687122-100
100 µl
$275
LS-C687122-200
200 µl
$315
LS-C687122-1
1 ml
$590
LS-C687122-10
10 ml
$2,370
Description
CEBPD antibody LS-C687122 is a biotin-conjugated rabbit polyclonal antibody to CEBPD (C / EBP Delta) from mouse and rat. Validated for IHC and WB.
Target
Mouse C/EBP Delta / CEBPD
Host
Rabbit
Reactivity
Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Biotin. Also available Unconjugated or conjugated with FITC, Cy3, HRP, APC, PE, APC, Cy7.
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Modifications
Unmodified
Applications
  • IHC
  • Western blot
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
CEBPd, Mouse
Specificity
The antibody is a rabbit polyclonal antibody raised against CEBPd. It has been selected for its ability to recognize CEBPd in immunohistochemical staining and western blotting.
Presentation
0.01 M PBS, pH 7.4, 0.05% ProClin™ 300, 50% Glycerol
Storage
Avoid repeated freeze/thaw cycles. Store at 4°C for frequent use. Aliquot and store at -20°C for 12 months.
Restrictions
For research use only.
About C/EBP Delta / CEBPD
P49716 NM_005195 NP_005186.2

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Anti-C/EBPd Antibody - Western Blot. Western blot of Anti-C/EBPd Antibody Lane 1: untreated wild-type (WT) cells Lane 2: WT cells treated 3 h with LPS Lane 3: C/EBPd-knock-out cells treated 3H with LPS Load: 35 ug per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4C Blocking: buffer TBST ECL was used for detection of endogenous protein in whole cell extracts from mouse primary macrophage cells. Predicted/Observed size: 28.8 kD, ~35 kD.
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Western Blot of Anti-C/EBPd Antibody Lane 1: untreated wild-type (WT) cells Lane 2: WT cells treated 3 h with LPS Lane 3: C/EBPd-knock-out cells treated 3H with LPS Load: 35 µg per lane Primary: Anti-C/EBPd Antibody used at a dilution of 1:15k overnight at 4°C Blocking: buffer TBST ECL was used for detection of endogenous protein in whole cell extracts from mouse primary macrophage cells. Predicted/Observed size: 28.8 kDa, ~35 kDa
Species: Mouse, Human
Applications: IHC, Western blot, Immunoprecipitation, ELISA

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Images

Immunohistochemistry - Paraffin

DAB staining on fromalin fixed paraffin- embedded brain tissue
DAB staining on fromalin fixed paraffin- embedded brain tissue

Western blot

Western Blot; Sample: Rat Spleen lysate; Primary Ab: 1µg/ml Rabbit Anti-Mouse CEBPd Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Western Blot; Sample: Rat Spleen lysate; Primary Ab: 1µg/ml Rabbit Anti-Mouse CEBPd Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

Western blot

Western Blot; Sample: Recombinant protein.
Western Blot; Sample: Recombinant protein.

Immunohistochemistry - Paraffin

DAB staining on fromalin fixed paraffin- embedded brain tissue
DAB staining on fromalin fixed paraffin- embedded brain tissue

Western blot

Western Blot; Sample: Rat Spleen lysate; Primary Ab: 1µg/ml Rabbit Anti-Mouse CEBPd Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Western Blot; Sample: Rat Spleen lysate; Primary Ab: 1µg/ml Rabbit Anti-Mouse CEBPd Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

Western blot

Western Blot; Sample: Recombinant protein.
Western Blot; Sample: Recombinant protein.

Immunohistochemistry - Paraffin

DAB staining on fromalin fixed paraffin- embedded brain tissue
DAB staining on fromalin fixed paraffin- embedded brain tissue

Western blot

Western Blot; Sample: Rat Spleen lysate; Primary Ab: 1µg/ml Rabbit Anti-Mouse CEBPd Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Western Blot; Sample: Rat Spleen lysate; Primary Ab: 1µg/ml Rabbit Anti-Mouse CEBPd Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

Western blot

Western Blot; Sample: Recombinant protein.
Western Blot; Sample: Recombinant protein.

Immunohistochemistry - Paraffin

DAB staining on fromalin fixed paraffin- embedded brain tissue
DAB staining on fromalin fixed paraffin- embedded brain tissue

Western blot

Western Blot; Sample: Rat Spleen lysate; Primary Ab: 1µg/ml Rabbit Anti-Mouse CEBPd Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
Western Blot; Sample: Rat Spleen lysate; Primary Ab: 1µg/ml Rabbit Anti-Mouse CEBPd Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

Western blot

Western Blot; Sample: Recombinant protein.
Western Blot; Sample: Recombinant protein.

Requested From: United States
Date Requested: 12/14/2018
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