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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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C/EBP Alpha / CEBPA Antibody (aa304‑330) LS‑C168648
CEBPA antibody LS-C168648 is an unconjugated rabbit polyclonal antibody to CEBPA (C / EBP Alpha) from human and mouse. Validated for Flow and WB.
Catalog
Size
Price
LS-C168648-400
400 µl
$305

Popular C/EBP Alpha / CEBPA Products

Anti-CEBPA / CEBP Alpha antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4685 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Bovine, Dog, Guinea pig, Pig, Rabbit, Chicken
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human, Mouse
Applications: Western blot, Flow Cytometry, ELISA
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Species: Human, Mouse
Applications: Western blot, Flow Cytometry, ELISA
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Product Description

CEBPA antibody LS-C168648 is an unconjugated rabbit polyclonal antibody to CEBPA (C / EBP Alpha) from human and mouse. Validated for Flow and WB.
About C/EBP Alpha / CEBPA
This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. P49715 NM_004364 NP_004355.2

CEBPA Antibody, C/EBP alpha Antibody, C/EBP-alpha Antibody, CEBP Antibody


Specifications

Target
Human C/EBP Alpha / CEBPA
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Rat, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Blocking Peptide
LS-E10153 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C168648. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa304-330
Specificity
This CEBPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-330 amino acids from the C-terminal region of human CEBPA.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.

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Images

Western blot

Western blot of CEBPA Antibody in mouse liver tissue lysates (35 ug/lane). CEBPA (arrow) was detected using the purified antibody.
Western blot of CEBPA Antibody in mouse liver tissue lysates (35 ug/lane). CEBPA (arrow) was detected using the purified antibody.

Flow Cytometry

CEBPA Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CEBPA Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot

Western blot of CEBPA Antibody in mouse liver tissue lysates (35 ug/lane). CEBPA (arrow) was detected using the purified antibody.
Western blot of CEBPA Antibody in mouse liver tissue lysates (35 ug/lane). CEBPA (arrow) was detected using the purified antibody.

Flow Cytometry

CEBPA Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CEBPA Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot

Western blot of CEBPA Antibody in mouse liver tissue lysates (35 ug/lane). CEBPA (arrow) was detected using the purified antibody.
Western blot of CEBPA Antibody in mouse liver tissue lysates (35 ug/lane). CEBPA (arrow) was detected using the purified antibody.

Flow Cytometry

CEBPA Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CEBPA Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot

Western blot of CEBPA Antibody in mouse liver tissue lysates (35 ug/lane). CEBPA (arrow) was detected using the purified antibody.
Western blot of CEBPA Antibody in mouse liver tissue lysates (35 ug/lane). CEBPA (arrow) was detected using the purified antibody.

Flow Cytometry

CEBPA Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CEBPA Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 9/26/2018

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