Products
Research Areas
COVID-19
Resources
Contact Us
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Cart
How To Buy
Contact Us

Locations

Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers


Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us


Additional Contact Details

Distributors
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Antibodies > BTG2 > LS-C662536
 
Print Page  
Catalog Number Size Price
LS-C662536-10 10 µg $318 
LS-C662536-100 100 µg $470 
BTG2 Antibody - Western blot analysis of BTG2 using anti-BTG2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates,Lane 2: rat brain tissue lysates,Lane 3: rat kidney tissue lysates,Lane 4: mouse brain tissue lysates,Lane 5: mouse brain tissue lysates,Lane 6: mouse kidney tissue lysates,Lane 7: human MDA-MB-231 whole cell lysates, Lane 8: human MDA-MB-453 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BTG2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BTG2 at approximately 17KD. The expected band size for BTG2 is at 17KD.
BTG2 Antibody - Western blot - Anti-BTG2 Picoband antibody
BTG2 Antibody - Flow Cytometry analysis of U20S cells using anti-BTG2 antibody. Overlay histogram showing U20S cells stained with anti-BTG2 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BTG2 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
BTG2 Antibody - Western blot analysis of BTG2 using anti-BTG2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates,Lane 2: rat brain tissue lysates,Lane 3: rat kidney tissue lysates,Lane 4: mouse brain tissue lysates,Lane 5: mouse brain tissue lysates,Lane 6: mouse kidney tissue lysates,Lane 7: human MDA-MB-231 whole cell lysates, Lane 8: human MDA-MB-453 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BTG2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BTG2 at approximately 17KD. The expected band size for BTG2 is at 17KD.
BTG2 Antibody - Western blot - Anti-BTG2 Picoband antibody
BTG2 Antibody - Flow Cytometry analysis of U20S cells using anti-BTG2 antibody. Overlay histogram showing U20S cells stained with anti-BTG2 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BTG2 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
1 of 3
2 of 3
3 of 3

Polyclonal Rabbit anti‑Human BTG2 Antibody (WB) LS‑C662536

Polyclonal Rabbit anti‑Human BTG2 Antibody (WB) LS‑C662536

Submit Review LSBio Guarantee
Antibody:
BTG2 Rabbit anti-Human Polyclonal Antibody
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662536-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
BTG2 Rabbit anti-Human Polyclonal Antibody
Application:
WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
BTG2 antibody LS-C662536 is an unconjugated rabbit polyclonal antibody to human BTG2. Validated for WB.
Target
Human BTG2
Synonyms
BTG2 | BTG family member 2 | BTG family, member 2 | PC3 | Pheochromacytoma cell-3 | Protein BTG2 | B-cell translocation gene 2 | TIS21
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence of human BTG2 (EQRLKVFSGALQEALTEHYKHHWFPEK).
Applications
  • Western blot
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BTG2
P78543 NM_006763 NP_006754.1

Publications (0)

Submit a Publication for LS-C662536

Customer Reviews (0)

Submit a review for LS-C662536

Featured Products

BTG2 Antibody - Fetal Lung Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
BTG2 Antibody (aa72‑121) LS‑C29836
Species: Human
Applications: Western blot
BTG2 Antibody - BTG2 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human prostate carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
BTG2 Antibody (aa33‑61) LS‑C156884
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
BTG2 Antibody - IHC of paraffin-embedded Human tonsil using anti-BTG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)(1:150).
BTG2 Antibody (clone OTI1G3) LS‑C340446
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
BTG2 Antibody - Western blot analysis of extracts of various cells.
BTG2 Antibody LS‑C411369
Species: Human
Applications: Western blot
HBV / HBsAg ELISA Kit
Human HBV / HBsAg (Sandwich ELISA) ELISA Kit - LS-F74868
Reactivity: Human
Range: 0.31-20 ng/ml
His Tag ELISA Kit
His Tag (Competitive EIA) ELISA Kit - LS-F55748
Reactivity: All species
Range: 24.69-2000 pg/ml
See all BTG2 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/27/2024
Family of Companies
Site Links Home All Antibodies How To Buy All Proteins Distributors All ELISA & Assay Kits Reviews PathPlus™ Antibodies
Follow LSBio View Cart Register or Login
Copyright © 2022 LifeSpan BioSciences, Inc - Privacy Policy
Contact Us

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

To place an order:
Orders@LSBio.com

For questions about orders:
Customer.Support@LSBio.com

For technical assistance:
Technical.Support@LSBio.com