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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BMP8B Antibody (clone AT13E6) LS‑C755409
BMP8B antibody LS-C755409 is an unconjugated mouse monoclonal antibody to human BMP8B. Validated for ELISA, ICC and WB.
Catalog
Size
Price
LS-C755409-50
50 µl (1 mg/ml)
$255
LS-C755409-100
100 µl (1 mg/ml)
$315
Description
BMP8B antibody LS-C755409 is an unconjugated mouse monoclonal antibody to human BMP8B. Validated for ELISA, ICC and WB.
Target
Human BMP8B
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [AT13E6]
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Applications
  • ICC (1:100)
  • Western blot (1:1000)
  • ELISA
Immunogen
BMP8B antibody was raised against recombinant human BMP8b (264-402aa) purified from E. coli
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 10% Glycerol
Restrictions
For research use only.
About BMP8B
P34820 NM_001720 NP_001711.2

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Images

Immunofluorescence

ICC/IF analysis of BMP8b in HaLe cells. The cell was stained with BMP8b antibody (1:100).The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
ICC/IF analysis of BMP8b in HaLe cells. The cell was stained with BMP8b antibody (1:100).The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP8b antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: BMP8b transfected 293T cell lysate The Human Recombinant protein BMP8b(200, 100, 50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP8bq antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP8b antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: BMP8b transfected 293T cell lysate The Human Recombinant protein BMP8b(200, 100, 50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP8bq antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunofluorescence

ICC/IF analysis of BMP8b in HaLe cells. The cell was stained with BMP8b antibody (1:100).The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
ICC/IF analysis of BMP8b in HaLe cells. The cell was stained with BMP8b antibody (1:100).The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP8b antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: BMP8b transfected 293T cell lysate The Human Recombinant protein BMP8b(200, 100, 50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP8bq antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP8b antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: BMP8b transfected 293T cell lysate The Human Recombinant protein BMP8b(200, 100, 50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP8bq antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunofluorescence

ICC/IF analysis of BMP8b in HaLe cells. The cell was stained with BMP8b antibody (1:100).The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
ICC/IF analysis of BMP8b in HaLe cells. The cell was stained with BMP8b antibody (1:100).The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP8b antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: BMP8b transfected 293T cell lysate The Human Recombinant protein BMP8b(200, 100, 50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP8bq antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP8b antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: BMP8b transfected 293T cell lysate The Human Recombinant protein BMP8b(200, 100, 50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP8bq antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunofluorescence

ICC/IF analysis of BMP8b in HaLe cells. The cell was stained with BMP8b antibody (1:100).The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
ICC/IF analysis of BMP8b in HaLe cells. The cell was stained with BMP8b antibody (1:100).The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Western blot

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP8b antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: BMP8b transfected 293T cell lysate The Human Recombinant protein BMP8b(200, 100, 50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP8bq antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP8b antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: BMP8b transfected 293T cell lysate The Human Recombinant protein BMP8b(200, 100, 50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP8bq antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Requested From: United States
Date Requested: 12/18/2018
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