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Catalog Number Size Price
LS-C748521-20 20 µl $275 
LS-C748521-100 100 µl $389 
BMI1 / PCGF4 Antibody - Immunofluorescence analysis of HeLa cells using BMI1 antibody. Blue: DAPI for nuclear staining.
BMI1 / PCGF4 Antibody - Western blot analysis of extracts of U-937 cells, using BMI1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
BMI1 / PCGF4 Antibody - Immunofluorescence analysis of HeLa cells using BMI1 antibody. Blue: DAPI for nuclear staining.
BMI1 / PCGF4 Antibody - Western blot analysis of extracts of U-937 cells, using BMI1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.
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Polyclonal Rabbit anti‑Human BMI1 / PCGF4 Antibody (IF, WB) LS‑C748521

Polyclonal Rabbit anti‑Human BMI1 / PCGF4 Antibody (IF, WB) LS‑C748521

Antibody:
BMI1 / PCGF4 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C748521-20
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
BMI1 / PCGF4 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
PCGF4 antibody LS-C748521 is an unconjugated rabbit polyclonal antibody to PCGF4 (BMI1) from human. It is reactive with human and mouse. Validated for IF and WB.
Target
Human BMI1 / PCGF4
Synonyms
BMI1 | BMI-1 | Flvi-2/bmi-1 | FLVI2/BMI1 | PCGF4 | Polycomb group protein Bmi1 | Polycomb complex protein BMI-1 | Dna-binding protein bmi-1 | Polycomb group ring finger 4 | RING finger protein 51 | RNF51
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 120-326 of human BMI1 (NP_005171.4). EDKRIITDDEIISLSIEFFDQNRLDRKVNKDKEKSKEEVNDKRYLRCPAAMTVMHLRKFLRSKMDIPNTFQIDVMYEEEPLKDYYTLMDIAYIYTWRRNGPLPLKYRVRPTCKRMKISHQRDGLTNAGELESDSGSDKANSPAGGIPSTSSCLPSPSTPVQSPHPQFPHISSTMNGTSNSPSGNHQSSFANRPRKSSVNGSSATSSG
Applications
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Usage
The predicted MW is 36kDa, while the observed MW by Western blot was 37kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BMI1 / PCGF4
P35226 NM_005180 NP_005171.4

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/29/2024