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Catalog Number Size Price
LS-C392812-200 200 µl $393 
BMI1 / PCGF4 Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human cervical epithelial adenocarcinoma cell line) cells labeling BMI1 with antibody at 1:25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1:400 dilution (green). Confocal image showing nuclear staining on HeLa cell line. Cytoplasmic actin is detected with Alexa Fluor 555 conjugated with Phalloidin (OB16636430) at 1:100 dilution (red).
BMI1 / PCGF4 Antibody - All lanes: Anti-BMI1 Antibody (C-term) at 1:1000-1:2000 dilution. Lane 1: A549 whole cell lysate. Lane 2: HeLa whole cell lysate. Lane 3: mouse thymus lysate. Lane 4: MCF-7 whole cell lysate. Lane 5: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 37 kDa. . Blocking/Dilution buffer: 5% NFDM/TBST.
BMI1 / PCGF4 Antibody - Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human cervical epithelial adenocarcinoma cell line) cells labeling BMI1 with antibody at 1:25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1:400 dilution (green). Confocal image showing nuclear staining on HeLa cell line. Cytoplasmic actin is detected with Alexa Fluor 555 conjugated with Phalloidin (OB16636430) at 1:100 dilution (red).
BMI1 / PCGF4 Antibody - All lanes: Anti-BMI1 Antibody (C-term) at 1:1000-1:2000 dilution. Lane 1: A549 whole cell lysate. Lane 2: HeLa whole cell lysate. Lane 3: mouse thymus lysate. Lane 4: MCF-7 whole cell lysate. Lane 5: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 37 kDa. . Blocking/Dilution buffer: 5% NFDM/TBST.
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Polyclonal Rabbit anti‑Human BMI1 / PCGF4 Antibody (aa281‑314, IF, WB) LS‑C392812

Polyclonal Rabbit anti‑Human BMI1 / PCGF4 Antibody (aa281‑314, IF, WB) LS‑C392812

Antibody:
BMI1 / PCGF4 Rabbit anti-Human Polyclonal (aa281-314) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C392812-200
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
BMI1 / PCGF4 Rabbit anti-Human Polyclonal (aa281-314) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
PCGF4 antibody LS-C392812 is an unconjugated rabbit polyclonal antibody to PCGF4 (BMI1) (aa281-314) from human. It is reactive with human and mouse. Validated for IF, Peptide-ELISA and WB.
Target
Human BMI1 / PCGF4
Synonyms
BMI1 | BMI-1 | Flvi-2/bmi-1 | FLVI2/BMI1 | PCGF4 | Polycomb group protein Bmi1 | Polycomb complex protein BMI-1 | Dna-binding protein bmi-1 | Polycomb group ring finger 4 | RING finger protein 51 | RNF51
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
aa281-314
Specificity
This BMI1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 281-314 amino acids from the C-terminal region of human BMI1.
Applications
  • Immunofluorescence (1:25)
  • Western blot (1:1000 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BMI1 / PCGF4
P35226 NM_005180 NP_005171.4

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Requested From: United States
Date Requested: 5/17/2024