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BLZF1 Antibody LS‑C668267

Catalog Number Size Price
LS-C668267-50 50 µl (1 mg/ml) $235 
LS-C668267-100 100 µl (1 mg/ml) $275 
Immunohistochemistry - Paraffin Image
Immunofluorescence Image
Western blot Image
Immunohistochemistry - Paraffin Image
Immunofluorescence Image
Western blot Image
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BLZF1 Antibody LS‑C668267

Antibody:
Rabbit Polyclonal to Human BLZF1
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$235 
LS-C668267-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human BLZF1
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
BLZF1 antibody LS-C668267 is an unconjugated rabbit polyclonal antibody to human BLZF1. Validated for ICC, IF, IHC and WB.
Target
Human BLZF1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity chromatography
Modifications
Unmodified
Applications
  • IHC (1:50 - 1:200)
  • IHC - Paraffin
  • ICC
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant full length protein of human BLZF1
Specificity
Recognizes endogenous levels of BLZF1 protein.
Usage
Western blot analysis of BLZF1 expression in MCF7 (A), NIH3T3 (B), Jurkat (C) whole cell lysates. Immunohistochemical analysis of BLZF1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Immunofluorescent analysis of BLZF1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Presentation
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BLZF1
Q9H2G9 NM_003666 NP_003657.1

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Requested From: United States
Date Requested: 9/23/2019