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Catalog Number Size Price
LS-C313475-10 10 µg $318 
LS-C313475-100 100 µg (0.5 mg/ml) $470 
BIRC5 / Survivin Antibody - Western blot analysis of Survivin using anti-Survivin antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse SP2/0 whole cell lysates, Lane 2: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Survivin antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Survivin at approximately 16KD. The expected band size for Survivin is at 16KD.
BIRC5 / Survivin Antibody - WB of BIRC5 / Survivin antibody. Lane 1: Recombinant Human Survivin Protein 10ng. Lane 2: Recombinant Human Survivin Protein 5ng. Lane 3: Recombinant Human Survivin Protein 2.5ng..
BIRC5 / Survivin Antibody - Western blot analysis of Survivin using anti-Survivin antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse SP2/0 whole cell lysates, Lane 2: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Survivin antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Survivin at approximately 16KD. The expected band size for Survivin is at 16KD.
BIRC5 / Survivin Antibody - WB of BIRC5 / Survivin antibody. Lane 1: Recombinant Human Survivin Protein 10ng. Lane 2: Recombinant Human Survivin Protein 5ng. Lane 3: Recombinant Human Survivin Protein 2.5ng..
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Polyclonal Rabbit anti‑Human BIRC5 / Survivin Antibody (aa1‑142, IHC, WB) LS‑C313475

Polyclonal Rabbit anti‑Human BIRC5 / Survivin Antibody (aa1‑142, IHC, WB) LS‑C313475

Antibody:
BIRC5 / Survivin Rabbit anti-Human Polyclonal (aa1-142) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C313475-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
BIRC5 / Survivin Rabbit anti-Human Polyclonal (aa1-142) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Survivin antibody LS-C313475 is an unconjugated rabbit polyclonal antibody to human Survivin (BIRC5) (aa1-142). Validated for ELISA, IHC and WB.
Target
Human BIRC5 / Survivin
Synonyms
BIRC5 | API4 | Apoptosis inhibitor survivin | Apoptosis inhibitor 4 | Survivin | Survivin variant 3 alpha | EPR-1 | IAP4
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived human Survivin recombinant protein(Position: M1-D142).
Epitope
aa1-142
Specificity
Expressed only in fetal kidney and liver, and to lesser extent, lung and brain. Abundantly expressed in adenocarcinoma (lung, pancreas, colon, breast, and prostate) and in high-grade lymphomas. Also expressed in various renal cell carcinoma cell lines. .
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BIRC5 / Survivin
O15392 NM_001168 NP_001159.2

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 6/6/2024