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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BHMT Antibody (clone 3E11) IHC‑plus™ LS‑B10964
Note: This antibody replaces LS-C115013
BHMT antibody LS-B10964 is an unconjugated mouse monoclonal antibody to human BHMT. Validated for Flow, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B10964-50
50 µl (0.93 mg/ml)
$425
Description
BHMT antibody LS-B10964 is an unconjugated mouse monoclonal antibody to human BHMT. Validated for Flow, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human BHMT
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal [3E11]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence (1:100)
  • Western blot (1:500)
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
BHMT antibody was raised against full length human recombinant protein of human BHMT (NP_001704) produced in HEK293T cell.
Specificity
Human BHMT
Usage
Concentration: 0.5-1.0 mg/ml (Lot dependent)
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About BHMT
Q93088 NM_001713 NP_001704.2

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Immunohistochemistry

Anti-BHMT antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-BHMT antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Immunofluorescence

Anti-BHMT mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BHMT.
Anti-BHMT mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BHMT.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BHMT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BHMT.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BHMT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BHMT.

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY BHMT (Red) or empty vector control plasmid (Blue) were immunostained with anti-BHMT mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY BHMT (Red) or empty vector control plasmid (Blue) were immunostained with anti-BHMT mouse monoclonal, and then analyzed by flow cytometry.

Immunohistochemistry

Anti-BHMT antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-BHMT antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Immunofluorescence

Anti-BHMT mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BHMT.
Anti-BHMT mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BHMT.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BHMT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BHMT.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BHMT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BHMT.

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY BHMT (Red) or empty vector control plasmid (Blue) were immunostained with anti-BHMT mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY BHMT (Red) or empty vector control plasmid (Blue) were immunostained with anti-BHMT mouse monoclonal, and then analyzed by flow cytometry.

Immunohistochemistry

Anti-BHMT antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-BHMT antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Immunofluorescence

Anti-BHMT mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BHMT.
Anti-BHMT mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BHMT.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BHMT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BHMT.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BHMT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BHMT.

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY BHMT (Red) or empty vector control plasmid (Blue) were immunostained with anti-BHMT mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY BHMT (Red) or empty vector control plasmid (Blue) were immunostained with anti-BHMT mouse monoclonal, and then analyzed by flow cytometry.

Immunohistochemistry

Anti-BHMT antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-BHMT antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Immunofluorescence

Anti-BHMT mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BHMT.
Anti-BHMT mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BHMT.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BHMT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BHMT.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BHMT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BHMT.

Flow Cytometry

HEK293T cells transfected with either pCMV6-ENTRY BHMT (Red) or empty vector control plasmid (Blue) were immunostained with anti-BHMT mouse monoclonal, and then analyzed by flow cytometry.
HEK293T cells transfected with either pCMV6-ENTRY BHMT (Red) or empty vector control plasmid (Blue) were immunostained with anti-BHMT mouse monoclonal, and then analyzed by flow cytometry.

Requested From: United States
Date Requested: 12/9/2018
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