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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Beta Tubulin Antibody (C‑term) LS‑C387404
Beta Tubulin antibody LS-C387404 is an unconjugated mouse monoclonal antibody to zebrafish Beta Tubulin. Validated for WB.
Catalog
Size
Price
LS-C387404-100
100 µl (1 mg/ml)
$265

Popular Beta Tubulin Products

Anti-Beta Tubulin antibody IHC of human kidney, glomeruli. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7936 dilution 10 ug/ml.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus
Applications: IHC, IHC - Paraffin, Western blot
Human Colon, MALT: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species:
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Immunohistochemical analysis of Beta-tubulin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Immunofluorescence analysis of beta Tubulin was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Beta Tubulin (TU-02) Rabbit Polyclonal Antibody at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic and nuclear localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Species: Pig
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Antibody
Species: Pig, Human, Mouse, Plant
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, ELISA

Product Description

Beta Tubulin Antibody (C-term) for WB/Western LS-C387404

Specifications

Target
Zebrafish Beta Tubulin
Host
Mouse
Reactivity
Zebrafish (tested or 100% immunogen sequence identity)
Clonality
Monoclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:2000 - 1:5000)
Immunogen
Beta Tubulin antibody was raised against synthetic peptide
Epitope
C-term
Specificity
The antibody detects Zebrafish endogenousbeta-tubulin protein.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Images

Western blot

Western blot of beta-Tubulin antibody
Western blot of beta-Tubulin antibody

Western blot

Western blot of beta-Tubulin antibody
Western blot of beta-Tubulin antibody

Western blot

Western blot of beta-Tubulin antibody
Western blot of beta-Tubulin antibody

Western blot

Western blot of beta-Tubulin antibody
Western blot of beta-Tubulin antibody

Requested From: United States
Date Requested: 10/20/2018

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