Human Beta Amyloid 1-40

Mouse

Human, Mouse (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Ab40.1

Unconjugated

Protein G purified

Unmodified

Synthetic peptide corresponding to Aß35-40.

• IHC (1 - 10 µg/ml)
• Immunofluorescence (1 - 10 µg/ml)
• Western blot (1 - 10 µg/ml)
• Immunoprecipitation (1 - 10 µg/ml)
• ELISA
• Sandwich ELISA

Immunoblotting, Immunohistochemistry: Immunofluorescence, Immunoprecipitation, Test at 1-10ug/ml. Sandwich ELISA (works equally well as capture or detection antibody). These are recommended concentrations; enduser should determine optimal concentrations for their applications. 96-well ELISA plates (Immulon HBX4) are coated with capture MAb at 2.5-5ug/well and incubated at 4oC overnight. The next day 300ul of blocking buffer is added, and plates are again incubated at 4oC overnight. The next day plates are washed in PBS and serial dilution of Aß samples are added; plates are incubated overnight at 4oC. The next day plates are washed in PBS, and HRP-conjugated detection Aß MAb is added; plates are incubated for 4hrs at room temperature. Plates are washed in PBS-Tween and developed with TMB substrate for 5 mins.

PBS, pH 7.4

Store at -20°C for up to 1 year.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.