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Antibodies > Basigin / Emmprin / CD147 > LS-C661880
 
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Catalog Number Size Price
LS-C661880-10 10 µg $318 
LS-C661880-100 100 µg $470 
Basigin / Emmprin / CD147 Antibody - IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody. CD147/Emmprin was detected in immunocytochemical section of HELA cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-CD147/Emmprin Antibody overnight at 4°C. DyLight®488 Conjugated Avidin was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1094) with DAB as the chromogen.
Basigin / Emmprin / CD147 Antibody - IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody. CD147/Emmprin was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-CD147/Emmprin Antibody overnight at 4°C. DyLight®488 Conjugated Avidin was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1094) with DAB as the chromogen.
Basigin / Emmprin / CD147 Antibody - IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody. CD147/Emmprin was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-CD147/Emmprin Antibody overnight at 4°C. DyLight®488 Conjugated Avidin was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1094) with DAB as the chromogen
Basigin / Emmprin / CD147 Antibody - Immunohistochemistry - Anti-CD147/Emmprin Picoband Antibody
Basigin / Emmprin / CD147 Antibody - Immunohistochemistry - Anti-CD147/Emmprin Picoband Antibody
Basigin / Emmprin / CD147 Antibody - Immunohistochemistry - Anti-CD147/Emmprin Picoband Antibody
Basigin / Emmprin / CD147 Antibody - IF analysis of CD147 using anti-CD147 antibody CD147 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-CD147 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Basigin / Emmprin / CD147 Antibody - Western blot analysis of CD147/Emmprin using anti-CD147/Emmprin antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Human Hela whole cell lysates, Lane 2: Human Placenta whole cell lysates, Lane 3: Human Jurkat whole cell lysates, Lane 4: Human U20S whole cell lysates, Lane 5: Human K562 whole cell lysates, Lane 6: Human MCF-7 whole cell lysates, Lane 7: Human COLO-320 whole cell lysates, Lane 8: Human HepG2 whole cell lysates, Lane 9: Human PANC-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD147/Emmprin antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD147/Emmprin at approximately 38KD and 55KD. The expected band size for CD147/Emmprin is at 42KD.
Basigin / Emmprin / CD147 Antibody - Western blot - Anti-CD147/Emmprin Picoband Antibody
Basigin / Emmprin / CD147 Antibody - Flow Cytometry analysis of Hela cells using anti-CD147/Emmprin antibody. Overlay histogram showing Hela cells stained with anti-CD147/Emmprin antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD147/Emmprin Antibody (1µg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Basigin / Emmprin / CD147 Antibody - Flow Cytometry analysis of A549 cells using anti-CD147/Emmprin antibody. Overlay histogram showing A549 cells stained with anti-CD147/Emmprin antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD147/Emmprin Antibody (1µg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Basigin / Emmprin / CD147 Antibody - IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody. CD147/Emmprin was detected in immunocytochemical section of HELA cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-CD147/Emmprin Antibody overnight at 4°C. DyLight®488 Conjugated Avidin was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1094) with DAB as the chromogen.
Basigin / Emmprin / CD147 Antibody - IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody. CD147/Emmprin was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-CD147/Emmprin Antibody overnight at 4°C. DyLight®488 Conjugated Avidin was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1094) with DAB as the chromogen.
Basigin / Emmprin / CD147 Antibody - IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody. CD147/Emmprin was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-CD147/Emmprin Antibody overnight at 4°C. DyLight®488 Conjugated Avidin was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1094) with DAB as the chromogen
Basigin / Emmprin / CD147 Antibody - Immunohistochemistry - Anti-CD147/Emmprin Picoband Antibody
Basigin / Emmprin / CD147 Antibody - Immunohistochemistry - Anti-CD147/Emmprin Picoband Antibody
Basigin / Emmprin / CD147 Antibody - Immunohistochemistry - Anti-CD147/Emmprin Picoband Antibody
Basigin / Emmprin / CD147 Antibody - IF analysis of CD147 using anti-CD147 antibody CD147 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-CD147 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Basigin / Emmprin / CD147 Antibody - Western blot analysis of CD147/Emmprin using anti-CD147/Emmprin antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Human Hela whole cell lysates, Lane 2: Human Placenta whole cell lysates, Lane 3: Human Jurkat whole cell lysates, Lane 4: Human U20S whole cell lysates, Lane 5: Human K562 whole cell lysates, Lane 6: Human MCF-7 whole cell lysates, Lane 7: Human COLO-320 whole cell lysates, Lane 8: Human HepG2 whole cell lysates, Lane 9: Human PANC-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD147/Emmprin antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD147/Emmprin at approximately 38KD and 55KD. The expected band size for CD147/Emmprin is at 42KD.
Basigin / Emmprin / CD147 Antibody - Western blot - Anti-CD147/Emmprin Picoband Antibody
Basigin / Emmprin / CD147 Antibody - Flow Cytometry analysis of Hela cells using anti-CD147/Emmprin antibody. Overlay histogram showing Hela cells stained with anti-CD147/Emmprin antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD147/Emmprin Antibody (1µg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Basigin / Emmprin / CD147 Antibody - Flow Cytometry analysis of A549 cells using anti-CD147/Emmprin antibody. Overlay histogram showing A549 cells stained with anti-CD147/Emmprin antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD147/Emmprin Antibody (1µg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human Basigin / Emmprin / CD147 Antibody (IHC, WB) LS‑C661880

Polyclonal Rabbit anti‑Human Basigin / Emmprin / CD147 Antibody (IHC, WB) LS‑C661880

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Antibody:
Basigin / Emmprin / CD147 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ICC, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C661880-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
Basigin / Emmprin / CD147 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ICC, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CD147 antibody LS-C661880 is an unconjugated rabbit polyclonal antibody to human CD147 (Basigin / Emmprin). Validated for ELISA, Flow, ICC, IHC and WB.
Target
Human Basigin / Emmprin / CD147
Synonyms
BSG | 5F7 | Basigin | Basigin (Ok blood group) | CD147 antigen | EMMPRIN | OK | OK blood group antigen | TCSF | CD147 | M6
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human CD147/Emmprin recombinant protein (Position: E138-A323). Human CD147/Emmprin shares 51.1% and 51.9% amino acid (aa) sequence identity with mouse and rat CD147/Emmprin, respectively.
Specificity
Present only in vascular endothelium in non- neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.
Applications
  • IHC
  • IHC - Paraffin
  • ICC
  • Western blot
  • Flow Cytometry
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Basigin / Emmprin / CD147
P35613 NM_001728 NP_001719.2

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Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/27/2024
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