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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C148222-50 50 µl $335 
BAR / BFAR Antibody - Formalin-fixed, paraffin-embedded tissue sections stained for BAR expression using Polyclonal Antibody to BAR at 1:2000. A. Two cores from a human glioblastoma tissue microarray: 1 =fibrillary astrocytoma (grade I), and2 = anaplastic glioma (grade III). B. Higher magnification from the fibrillary astrocytoma (shown in A). C. Higher magnification from the anaplastic glioma (shown in A). D. Normal human brain striatum with positive medium spiny neurons, the major neuronal cell type of the striatum. Hematoxylin-Eos in counterstain.
BAR / BFAR Antibody - Formalin-fixed, paraffin-embedded human prostate carcinoma tissue array stained for BAR expression using Polyclonal Antibody to BAR at 1:2000. Hematoxylin-Eosin counterstain. Variable BAR expression is seen between patient samples.
BAR / BFAR Antibody - Western blot of BAR in normal prostate and prostate carcinoma tissue lysates. 25 ug protein was loaded per lane. Tissue lysates from 15 different prostate carcinoma patients show variable expression of BAR with respect to banding patterns and amount of BAR expression. Major BAR bands typically migrate as a single band or as a doublet. These bands are typically observed at ~50-54 kD. This is higher than the predicted molecular weight from the 450 amino acid BAR sequence, and may represent phosphorylation or other post-translational modifications. N = tissue lysate from normal prostate.
BAR / BFAR Antibody - Formalin-fixed, paraffin-embedded tissue sections stained for BAR expression using Polyclonal Antibody to BAR at 1:2000. A. Two cores from a human glioblastoma tissue microarray: 1 =fibrillary astrocytoma (grade I), and2 = anaplastic glioma (grade III). B. Higher magnification from the fibrillary astrocytoma (shown in A). C. Higher magnification from the anaplastic glioma (shown in A). D. Normal human brain striatum with positive medium spiny neurons, the major neuronal cell type of the striatum. Hematoxylin-Eos in counterstain.
BAR / BFAR Antibody - Formalin-fixed, paraffin-embedded human prostate carcinoma tissue array stained for BAR expression using Polyclonal Antibody to BAR at 1:2000. Hematoxylin-Eosin counterstain. Variable BAR expression is seen between patient samples.
BAR / BFAR Antibody - Western blot of BAR in normal prostate and prostate carcinoma tissue lysates. 25 ug protein was loaded per lane. Tissue lysates from 15 different prostate carcinoma patients show variable expression of BAR with respect to banding patterns and amount of BAR expression. Major BAR bands typically migrate as a single band or as a doublet. These bands are typically observed at ~50-54 kD. This is higher than the predicted molecular weight from the 450 amino acid BAR sequence, and may represent phosphorylation or other post-translational modifications. N = tissue lysate from normal prostate.
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BAR / BFAR Antibody (aa3‑21) LS‑C148222

BAR / BFAR Antibody (aa3‑21) LS‑C148222

Antibody:
Rabbit Polyclonal to Human BAR / BFAR
Application:
IHC, IHC-P, IHC-Fr, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$335 
LS-C148222-50
50 µl
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human BAR / BFAR
Application:
IHC, IHC-P, IHC-Fr, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
BFAR antibody LS-C148222 is an unconjugated rabbit polyclonal antibody to human BFAR (BAR ). Validated for IHC, IP and WB.
Target
Human BAR / BFAR
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:1000 - 1:5000)
  • IHC - Frozen
  • Western blot (1:1000 - 1:2000)
  • Immunoprecipitation (1:50 - 1:200)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A synthetic peptide corresponding to amino acids 3-21 (EPQKSYVNTMDLERDEPLK) of human BAR, GenBank no. NP_057645.1. Human BAR is a 450 amino acid protein. Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon, Monkey (84%).
Epitope
aa3-21
Specificity
BAR.
Presentation
Antiserum, 0.05% sodium azide
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BAR / BFAR
Q9NZS9 NM_016561 NP_057645.1

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Requested From: United States
Date Requested: 11/12/2019